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Ziege anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (Atto 594) - Preadsorbed

WB, FLISA, FM Polyclonal IgG Atto 594
Produktnummer ABIN964988
  • Target Alle IgG Produkte
    IgG
    Bindungsspezifität
    • 2333
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reaktivität
    • 695
    • 672
    • 561
    • 458
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
    • 97
    • 95
    • 92
    • 62
    • 47
    • 38
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Kaninchen
    Wirt
    • 1730
    • 1474
    • 539
    • 206
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Ziege
    Klonalität
    • 3361
    • 167
    • 1
    Polyklonal
    Konjugat
    • 557
    • 409
    • 406
    • 368
    • 298
    • 276
    • 228
    • 121
    • 99
    • 67
    • 66
    • 62
    • 62
    • 52
    • 48
    • 47
    • 45
    • 44
    • 44
    • 41
    • 39
    • 34
    • 33
    • 33
    • 31
    • 31
    • 30
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 26
    • 26
    • 25
    • 21
    • 19
    • 17
    • 17
    • 16
    • 16
    • 15
    • 15
    • 14
    • 10
    Atto 594
    Applikation
    • 1840
    • 1800
    • 1792
    • 1501
    • 962
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 52
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
    Spezifität
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
    Produktmerkmale
    Anti-Rabbit IgG (H&L) conjugated to ATTO 594 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Aufreinigung
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Rabbit IgG whole molecule

    Isotyp
    IgG
    Labeling Ratio
    2.5
  • Applikationshinweise

    Application Note: Anti-Rabbit IgG (H&L) conjugated to ATTO 594 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

    FLISA Dilution: >1:20,000

    Western Blot Dilution: >1:10,000

    IF Microscopy Dilution: >1:5,000

    Kommentare

    The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid cycles of freezing and thawing.
    Product is photosensitive and should be protected from light.
    Lagerung
    RT,4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
    Haltbarkeit
    12 months
  • Kummer, Avinoam, Kräusslich: "IFITM3 Clusters on Virus Containing Endosomes and Lysosomes Early in the Influenza A Infection of Human Airway Epithelial Cells." in: Viruses, Vol. 11, Issue 6, (2019) (PubMed).

    Purkey, Woolfrey, Crosby, Stich, Chick, Aoto, DellAcqua: "AKAP150 Palmitoylation Regulates Synaptic Incorporation of Ca2+-Permeable AMPA Receptors to Control LTP." in: Cell reports, Vol. 25, Issue 4, pp. 974-987.e4, (2018) (PubMed).

    Coffman, Kachur, Pilgrim, Dawes: "Antagonistic Behaviors of NMY-1 and NMY-2 Maintain Ring Channels in the C. elegans Gonad." in: Biophysical journal, Vol. 111, Issue 10, pp. 2202-2213, (2017) (PubMed).

  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund

    Synonyms: Goat anti-Rabbit IgG Antibody ATTO594 Conjugation, Goat anti-Rabbit IgG ATTO 594 Conjugated Antibody

    Background: Anti-Rabbit IgG (H&L) ATTO 594 Antibody generated in goat detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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