Ziege anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (Atto 488)

Details zu Produkt Nr. ABIN964982, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Heavy & Light Chain
6508
2071
2051
1189
814
645
311
269
33
30
30
15
12
12
12
12
11
7
3
2
1
1
1
1
1
1
1
Reaktivität
Kaninchen
3172
3055
2510
1746
1580
820
766
504
483
430
411
409
335
291
277
173
159
128
54
30
30
26
15
12
12
8
7
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Wirt
Ziege
7494
5381
2124
1082
552
357
89
73
55
35
23
15
8
5
1
1
1
Klonalität
Polyklonal
Konjugat
Atto 488
2298
1890
1829
1326
792
657
536
340
269
174
147
145
143
140
119
112
112
102
98
94
93
91
89
86
84
81
79
78
75
73
68
68
65
61
55
54
54
54
54
53
53
53
53
53
53
52
49
48
43
41
38
36
32
31
23
23
22
20
19
17
16
15
15
14
14
14
12
12
12
12
11
11
11
10
10
10
10
9
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Applikation
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen

Immunogen: Rabbit IgG whole molecule

Isotyp IgG
Spezifität Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Kreuzreaktivität (Details) Pre-Adsorbed
Produktmerkmale Anti-Rabbit IgG (H&L) conjugated to ATTO 488 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reinigung Rabbit IgG (H&L) Antibody ATTO 488 was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.  This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.
Labeling Ratio 3.0
Antigen
Hintergrund

Synonyms: Goat anti-Rabbit IgG Antibody ATTO488 Conjugation, Goat anti-Rabbit IgG ATTO 488 Conjugated Antibody

Background: Anti-Rabbit IgG (H&L) ATTO 488 Antibody generated in goat detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Applikations-hinweise

Application Note: Anti-Rabbit IgG (H&L) conjugated to ATTO 488 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Kommentare

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Product is photosensitive and should be protected from light.
Lagerung RT,4 °C,-20 °C
Informationen zur Lagerung Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 488) (ABIN964982) Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 488)
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 488) (ABIN964982) Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs,...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 488) (ABIN964982) Western Blot of Goat anti-Rabbit IgG Pre-Adsorbed Atto 488 Conjugated Secondary Antib...