RFP Antikörper (Red Fluorescent Protein) (AA 234)

Details for Product anti-RFP Antibody No. ABIN964932, Anbieter: Anmelden zum Anzeigen
Antigen
  • RFP
  • RNF76
  • tripartite motif containing 27
  • TRIM27
Epitop
AA 234
28
6
3
2
1
Reaktivität
Discosoma
81
50
37
9
7
1
1
Wirt
Huhn
94
38
14
8
1
Klonalität
Polyklonal
Konjugat
Dieser RFP Antikörper ist unkonjugiert
9
7
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
132
67
60
53
32
27
6
5
4
2
2
1
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Immunogen The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Immunogen Type: RecombinantProtein
Isotyp IgG
Spezifität This product was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above.  Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum.
Produktmerkmale Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS). This product shows optimal performance by western blot.
Reinigung affinity purified
Sterilität Sterile filtered
Andere Bezeichnung RFP (RFP Antibody Abstract)
Hintergrund Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.

Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583.
Gen-ID 55976617
UniProt Q9U6Y8
Applikationshinweise Anti-RFP is designed to detect recombinant RFP. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.
Kommentare

Gene Name: DsRed

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (ABIN964932) Western blot of Anti-RFP Antibody. 0.1 µg of RFP was loaded on a 4-20% gel and tranfe...
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (ABIN964932) Western blot of Anti-RFP Antibody. 0.1 µg of RFP was loaded on a 4-20% gel and tranf...
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) (AA 234) antibody (ABIN964932) Western Blot Results of Chicken Anti-RFP Antibody. Lane 1: Opal PreStained Molecular ...
Produkt verwendet in: Haller, Hock, Giampazolias, Oberst, Green, Debnath, Ryan, Vousden, Tait: "Ubiquitination and proteasomal degradation of ATG12 regulates its proapoptotic activity." in: Autophagy, Vol. 10, Issue 12, pp. 2269-78, 2015 (PubMed).

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