SMAD3 Antikörper (Internal Region, pThr179)
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- Target Alle SMAD3 Antikörper anzeigen
- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
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Bindungsspezifität
- Internal Region, pThr179
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser SMAD3 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Spezifität
- This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pT179 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pT179 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad2. Reactivity against other phosphorylated Smad family members is not known. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
- Produktmerkmale
- This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.
- Aufreinigung
- affinity purified
- Sterilität
- Sterile filtered
- Immunogen
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This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.
Immunogen Type: Peptide - Isotyp
- IgG
- Top Product
- Discover our top product SMAD3 Primärantikörper
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- Applikationshinweise
- This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48.1 kDa in size corresponding to human phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
- Kommentare
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Gene Name: SMAD3
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.97 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.
- Haltbarkeit
- 3 months
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Discovery of a Small-Molecule BMP Sensitizer for Human Embryonic Stem Cell Differentiation." in: Cell reports, Vol. 15, Issue 9, pp. 2063-75, (2017) (PubMed).
: "Mediator kinase inhibition further activates super-enhancer-associated genes in AML. ..." in: Nature, Vol. 526, Issue 7572, pp. 273-6, (2015) (PubMed).
: "OTUB1 enhances TGFβ signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3." in: Nature communications, Vol. 4, pp. 2519, (2014) (PubMed).
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Discovery of a Small-Molecule BMP Sensitizer for Human Embryonic Stem Cell Differentiation." in: Cell reports, Vol. 15, Issue 9, pp. 2063-75, (2017) (PubMed).
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- Target
- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
- Andere Bezeichnung
- SMAD3 (SMAD3 Produkte)
- Synonyme
- HSPC193 antikoerper, HsT17436 antikoerper, JV15-2 antikoerper, LDS1C antikoerper, LDS3 antikoerper, MADH3 antikoerper, madh3 antikoerper, madh3a antikoerper, smad3 antikoerper, wu:fa99e03 antikoerper, XSmad3 antikoerper, XenMLP antikoerper, Xmad3 antikoerper, madh3-A antikoerper, Madh3 antikoerper, AU022421 antikoerper, Smad 3 antikoerper, mad3 antikoerper, madh3b antikoerper, zgc:92234 antikoerper, SMAD family member 3 antikoerper, SMAD family member 3a antikoerper, SMAD family member 3 L homeolog antikoerper, SMAD family member 3b antikoerper, SMAD3 antikoerper, smad3a antikoerper, smad3.L antikoerper, Smad3 antikoerper, smad3b antikoerper
- Hintergrund
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This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.
Synonyms: hMAD 3 antibody, hSMAD3 antibody, MADH3 antibody, MGC60396 antibody, Mothers against decapentaplegic homolog 3 antibody, Mothers against DPP homolog 3 antibody - Gen-ID
- 4088
- NCBI Accession
- NP_005893
- UniProt
- P84022
- Pathways
- Zellzyklus, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagie
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