CTCF Antikörper (C-Term)

Produktnummer ABIN964616

Produktdetails anti-CTCF Antikörper

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CTCF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with endogenous CTCF protein. A BLAST analysis was used to suggest reactivity with CTCF from human, mouse, horse, bovine, panda, rabbit, Danio, and chicken based on a 100% homology with the immunizing sequence. Cross-reactivity with CTCF from other sources has not been determined.

Kreuzreaktivität: Maus

Produktmerkmale: Transcriptional repressor CTCF (also known as CCCTC-binding factor) is a transcription factor encoded by the CTCF gene. The CTCF, and the closely related Brother of the Regulator of Imprinted Sites (BORIS), are highly conserved zinc finger proteins implicated in diverse regulatory functions, including transcriptional activation/repression, insulation, imprinting, and X chromosome inactivation. Expression of BORIS is restricted to spermatocytes and is mutually exclusive of CTCF expression. CTCF is ubiquitously expressed in higher eukaryotes and contains a highly conserved and eleven zinc finger central DNA-binding domain, having very high homology between mouse, chicken, and human and is embedded within slightly divergent N and C termini. CTFC plays a critical role in the epigenetic regulation and chromatin remodeling. CTCF has been reported to bind to a variety of DNA target sites that perform distinct functions, including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting.

Aufreinigung: affinity purified

Sterilität: Sterile filtered

Immunogen: This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of CTCF protein.
Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: CTCF antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 82.8 kDa or 150 kDa in size corresponding to CTCF by western blotting in the appropriate cell lysate or extract.

Kommentare:

Gene Name: CTCF

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.3 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen für anti-CTCF Antikörper (ABIN964616)

Majumder, Rupp, Yang-Iott, Koues, Kyle, Bassing, Oltz: "Domain-Specific and Stage-Intrinsic Changes in Tcrb Conformation during Thymocyte Development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 3, pp. 1262-72, (2015) (PubMed).

Details zu CTCF

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Andere Bezeichnung: CTCF (CTCF Produkte)

Synonyme: im:7152666 antikoerper, CTCF antikoerper, xctcf antikoerper, AW108038 antikoerper, CCCTC-binding factor antikoerper, CCCTC-binding factor (zinc finger protein) antikoerper, CTCF antikoerper, ctcf antikoerper, Ctcf antikoerper

Hintergrund: Transcriptional repressor CTCF (also known as CCCTC-binding factor) is a transcription factor encoded by the CTCF gene. The CTCF, and the closely related Brother of the Regulator of Imprinted Sites (BORIS), are highly conserved zinc finger proteins implicated in diverse regulatory functions, including transcriptional activation/repression, insulation, imprinting, and X chromosome inactivation. Expression of BORIS is restricted to spermatocytes and is mutually exclusive of CTCF expression. CTCF is ubiquitously expressed in higher eukaryotes and contains a highly conserved and eleven zinc finger central DNA-binding domain, having very high homology between mouse, chicken, and human and is embedded within slightly divergent N and C termini. CTFC plays a critical role in the epigenetic regulation and chromatin remodeling. CTCF has been reported to bind to a variety of DNA target sites that perform distinct functions, including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting.
Synonyms: Transcriptional repressor CTCF, 11-zinc finger protein, CCCTC-binding factor, CTCFL paralog

Gen-ID: 10664

NCBI Accession: NP_006556

UniProt: P49711