CEBPD Antikörper (N-Term)

Produktnummer ABIN964606

Produktdetails anti-CEBPD Antikörper

Target: CEBPD (CCAAT/enhancer Binding Protein (C/EBP), delta (CEBPD))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CEBPD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for mouse C/EBP delta protein. A BLAST analysis was used to suggest cross-reactivity with C/EBPd from human sources based on 100% homology with the immunizing sequence. No cross-reactivity is observed with mouse C/EBPa or C/EBPb. Reactivity with C/EBPd from other sources has not been determined.

Kreuzreaktivität: Human

Produktmerkmale: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression. Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T). Phosphorylation of C/EBPd may increase binding activity, whereas phosphorylation of the a and b family members may decrease binding affinity. C/EBPd is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.

Aufreinigung: purified

Sterilität: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse C/EBP.
Immunogen Type: RecombinantProtein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 35 kDa in size corresponding to C/EBPd by western blotting in the appropriate cell lysate or extract.   The predicted molecular weight of mouse C/EBPd is 28.8 kDa. This antibody is capable of detecting both over-expressed and endogenous C/EBPd.

Kommentare:

Gene Name: CEBPD

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.83 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is three (3) months from date of opening.

Haltbarkeit: 3 months

Referenzen für anti-CEBPD Antikörper (ABIN964606)

Puzio-Kuter, Laddha, Castillo-Martin, Sun, Cordon-Cardo, Chan, Levine: "Involvement of tumor suppressors PTEN and p53 in the formation of multiple subtypes of liposarcoma." in: Cell death and differentiation, Vol. 22, Issue 11, pp. 1785-91, (2015) (PubMed).

Sarkar, Sharan, Wang, Pawar, Cantwell, Johnson, Morrison, Wang, Sterneck: "Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBPδ expression and contributes to transformation of breast tumor cells." in: Molecular and cellular biology, Vol. 32, Issue 2, pp. 320-32, (2012) (PubMed).

Balamurugan, Wang, Tsai, Sharan, Anver, Leighty, Sterneck: "The tumour suppressor C/EBPδ inhibits FBXW7 expression and promotes mammary tumour metastasis." in: The EMBO journal, Vol. 29, Issue 24, pp. 4106-17, (2011) (PubMed).

Details zu CEBPD

Target: CEBPD (CCAAT/enhancer Binding Protein (C/EBP), delta (CEBPD))

Andere Bezeichnung: CEBP Delta (CEBPD Produkte)

Synonyme: C/EBP-delta antikoerper, CELF antikoerper, CRP3 antikoerper, NF-IL6-beta antikoerper, C/EBPd antikoerper, LOC398729 antikoerper, LOC398730 antikoerper, MGC114740 antikoerper, c/ebp-delta antikoerper, celf antikoerper, crp3 antikoerper, nf-il6-beta antikoerper, c/ebpd antikoerper, wu:fa99c04 antikoerper, wu:fb61h03 antikoerper, CEBPD antikoerper, cebpd antikoerper, CCAAT/enhancer binding protein (C/EBP), delta antikoerper, CCAAT/enhancer binding protein delta antikoerper, CCAAT/enhancer binding protein delta L homeolog antikoerper, CCAAT/enhancer binding protein delta S homeolog antikoerper, Cebpd antikoerper, CEBPD antikoerper, cebpd.L antikoerper, cebpd.S antikoerper, cebpd antikoerper

Hintergrund: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages.  Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression.  Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T).  Phosphorylation of C/EBPd may increase binding activity, whereas phosphorylation of the a and b family members may decrease binding affinity.  C/EBPd is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.
Synonyms: C/EBP related protein 3 antibody, CCAAT/enhancer binding protein delta antibody, CEBP D antibody, CEBPD antibody, CELF antibody, Crp 3 antibody, Crp3 antibody, NF IL6 beta antibody, Nuclear factor NF IL6 beta antibody

Gen-ID: 12609, 110347410

UniProt: Q00322