Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

MYB Antikörper (N-Term, pThr486)

MYB Reaktivität: Maus WB, ELISA Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN964595
  • Target Alle MYB Antikörper anzeigen
    MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))
    Bindungsspezifität
    • 15
    • 11
    • 11
    • 10
    • 8
    • 8
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term, pThr486
    Reaktivität
    • 117
    • 51
    • 8
    • 7
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    Maus
    Wirt
    • 96
    • 21
    • 3
    • 3
    Kaninchen
    Klonalität
    • 102
    • 21
    Polyklonal
    Konjugat
    • 79
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser MYB Antikörper ist unkonjugiert
    Applikation
    • 93
    • 57
    • 44
    • 24
    • 23
    • 13
    • 13
    • 12
    • 9
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA
    Spezifität
    This affinity-purified antibody is directed against the phosphorylated form of mouse c-Myb protein at the pT486 residue. Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.  Reactivity occurs against mouse c-Myb pT486 protein and the antibody is specific for the phosphorylated form of the protein.  This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells. Reactivity with non-phosphorylated mouse c-Myb is minimal by ELISA and western blot. A BLAST analysis was used to suggest reactivity with c-Myb from mouse based on a 100% homology with the immunizing sequence.  Expect reactivity with c-Myb from rat, pig, human, horse, platypus, opossum, orangutan, spider monkey, bonobo, gorilla, and chimpanzee, with a v-myb myeloblastosis viral oncogene homolog, all isoforms (also avian version) from human, and with a protooncogene c-myb, and a v-myb myeloblastosis viral oncogene homolog (avian), both from cattle, all based on a 93% homology with the immunizing sequence.  Cross-reactivity with c-Myb from other sources has not been determined.
    Produktmerkmale
    This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The transcription factor c-Myb plays an important role in the development of all hematopoietic lineages during definitive hematopoiesis. Its critical role in hematopoiesis has been first demonstrated in experiments with targeted disruption of the c-Myb gene where deletion of both alleles of the c-Myb gene was embryonally lethal due to severe anemia. c-Myb is a short-lived sequence specific DNA-binding protein that regulates transcription of several important genes involved directly in cellular processes such as proliferation, differentiation, and apoptosis. Post-translational modifications such as phosphorylation, acetylation, ubiquitination, and conjugation of SUMO proteins are crucial for modulation of the transactivation activity of c-Myb. Several phosphorylation sites serve to regulate c-Myb activity.
    Aufreinigung
    affinity purified
    Sterilität
    Sterile filtered
    Immunogen
    This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse c-Myb protein surrounding amino acid residue 486.
    Immunogen Type: Peptide
    Isotyp
    IgG
    Top Product
    Discover our top product MYB Primärantikörper
  • Applikationshinweise
    This affinity purified antibody has been tested for use in ELISA and western blotting using M1 cells which express endogenous wild-type c-Myb protein and in COS7 cells transfected with c-Myb.  Specific conditions for reactivity and detection of phosphorylated c-Myb should be optimized by the end user.  Expect a band approximately ~75 kDa in size corresponding to phosphorylated c-Myb by western blotting in the appropriate cell lysate or extract.
    Kommentare

    Gene Name: MYB

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.91 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    Haltbarkeit
    12 months
  • Target
    MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))
    Andere Bezeichnung
    C-MYB (MYB Produkte)
    Synonyme
    Cmyb antikoerper, c-myb antikoerper, c-myb_CDS antikoerper, efg antikoerper, AI550390 antikoerper, M16449 antikoerper, cmyb antikoerper, xmyb antikoerper, MYB proto-oncogene, transcription factor antikoerper, myeloblastosis oncogene antikoerper, MYB proto-oncogene, transcription factor S homeolog antikoerper, v-myb avian myeloblastosis viral oncogene homolog antikoerper, MYB antikoerper, Myb antikoerper, myb.S antikoerper, myb antikoerper
    Hintergrund
    This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. The transcription factor c-Myb plays an important role in the development of all hematopoietic lineages during definitive hematopoiesis. Its critical role in hematopoiesis has been first demonstrated in experiments with targeted disruption of the c-Myb gene where deletion of both alleles of the c-Myb gene was embryonally lethal due to severe anemia. c-Myb is a short-lived sequence specific DNA-binding protein that regulates transcription of several important genes involved directly in cellular processes such as proliferation, differentiation, and apoptosis. Post-translational modifications such as phosphorylation, acetylation, ubiquitination, and conjugation of SUMO proteins are crucial for modulation of the transactivation activity of c-Myb. Several phosphorylation sites serve to regulate c-Myb activity.
    Synonyms: Avian myeloblastosis viral (v-myb) oncogene homolog antibody, C myb antibody, MYB antibody, v-myb avian myeloblastosis viral oncogene homolog antibody
    Gen-ID
    17863, 110556654
    UniProt
    P06876
    Pathways
    Chromatin Binding, Positive Regulation of Immune Effector Process
Sie sind hier:
Kundenservice