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DYKDDDDK Tag Antikörper

WB, ELISA, IP Wirt: Maus Monoclonal 5A8E5 unconjugated
Produktnummer ABIN962815
  • Target Alle DYKDDDDK Tag Produkte
    DYKDDDDK Tag
    Reaktivität
    Bitte anfragen
    Wirt
    • 58
    • 52
    • 9
    • 8
    • 6
    Maus
    Klonalität
    • 67
    • 65
    Monoklonal
    Konjugat
    • 47
    • 15
    • 10
    • 7
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser DYKDDDDK Tag Antikörper ist unkonjugiert
    Applikation
    • 80
    • 62
    • 29
    • 24
    • 20
    • 19
    • 12
    • 12
    • 10
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Marke
    THE™
    Sequenz
    DYKDDDDK
    Spezifität
    THETM DYKDDDDK Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal and internal tagged fusion proteins.
    Produktmerkmale
    Mouse Anti-DYKDDDDK-tag Monoclonal Antibody is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins.
    Aufreinigung
    Protein A affinity column
    Immunogen
    A synthetic peptide (DYKDDDDK) coupled - KLH
    Klon
    5A8E5
    Isotyp
    IgG
  • Applikationshinweise
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.05-0.2 µg/mL
    Western blot: 0.1-1.0 µg/mL
    Immunoprecipitation: 1 µg/mL
    Other applications: user-optimized
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4, containing 0.02 % Sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handhabung
    Avoid repeated freezing and thawing cycles.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
  • Reuben, Phénix, Santos, Lapointe: "The WT hemochromatosis protein HFE inhibits CD8? T-lymphocyte activation." in: European journal of immunology, Vol. 44, Issue 6, pp. 1604-14, (2014) (PubMed).

    Maryon, Molloy, Kaplan: "Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1." in: American journal of physiology. Cell physiology, Vol. 304, Issue 8, pp. C768-79, (2013) (PubMed).

  • Target
    DYKDDDDK Tag
    Abstract
    DYKDDDDK Tag Produkte
    Substanzklasse
    Tag
    Hintergrund
    The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors.
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