Maus anti-Human IgM (Fc Region) Antikörper (FITC)

Details zu Produkt Nr. ABIN94403, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Fc Region
559
389
385
63
56
53
45
27
5
5
2
1
1
1
1
Reaktivität
Human
1218
585
242
96
86
76
74
64
62
62
60
43
40
28
20
19
19
11
5
5
2
1
1
1
Wirt
Maus
1309
677
582
144
41
17
10
4
3
3
3
1
Klonalität (Klon)
Monoklonal ()
Konjugat
FITC
377
365
288
142
98
84
67
52
41
23
21
20
20
20
20
20
18
16
16
15
13
12
9
7
6
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Purified human IgM.
Klon CH2
Isotyp IgG
Spezifität The antibody CH2 reacts with Fc fragment of human IgM.
Produktmerkmale The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Reinigung Purified from rabbit serum by protein-G affinity chromatography.
Hintergrund Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
Molekulargewicht 900 kDa
Applikationshinweise The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1: 500. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Working concentrations should be determined by the investigator.
Kommentare

Excitation/Emission wavelength: 494 nm/514 nm

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Do not freeze.
Avoid prolonged exposure to light.
Lagerung 4 °C
Informationen zur Lagerung Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Bilder des Herstellers
Flow Cytometry (FACS) image for Mouse anti-Human IgM (Fc Region) antibody (FITC) (ABIN94403) Surface staining of human peripheral blood cells with anti-human IgM (CH2) APC. Cells...
Allgemeine Veröffentlichungen Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." in: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." in: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." in: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).