Maus anti-Rind (Kuh) IgG (Light Chain) Antikörper

Details zu Produkt Nr. ABIN94401, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Light Chain
4782
2534
1961
1067
1022
860
306
292
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reaktivität
Rind (Kuh)
2897
2761
2297
1676
1453
790
626
453
395
387
359
346
298
296
221
138
135
130
41
31
29
24
16
11
10
8
7
6
5
5
4
4
4
3
3
3
3
3
3
3
2
2
1
1
1
1
1
Wirt
Maus
6786
4909
1788
922
609
392
91
68
55
18
8
5
5
5
2
1
1
Klonalität (Klon)
Monoklonal ()
Applikation
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Fraction of Bovine immunoglobulin.
Klon IVA285-1
Isotyp IgG1
Spezifität The antibody IVA285-1 reacts with bovine IgG (light chains). IgG light chains (kappa nad lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
Reinigung Purified from ascites by protein-A affinity chromatography.
Reinheit > 95 % (by SDS-PAGE)
Applikationshinweise Western Blotting: Recommended dilution: 0,5-1 µg/mL, overnight in 4 °C
Positive control and sample preparation: BS (bovine serum), dilution 1: 50 in Laemmli reducing buffer, boiled in water bath for 3 min
Application notes: Strongly reacts with bovine IgG light chains, weakly reacts with IgM.Reducing conditions. SDS-PAGE (12 % separating gel).
Flow Cytometry:

Working concentrations should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Bilder des Herstellers
Western Blotting (WB) image for Mouse anti-Cow (Bovine) IgG (Light Chain) antibody (ABIN94401) Detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and B...
Produkt verwendet in: Antalíková, Simon, Jankovicová, Horovská, Dusinský, Hluchý: "Monoclonal antibody to the light chain of bovine immunoglobulin." in: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, 2006 (PubMed).