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TUBG1 Antikörper (C-Term)

TUBG1 Reaktivität: Human, Ratte, Maus, Huhn, Rind (Kuh), Pflanzen, Protozoa, Schwein WB, ICC, FACS Wirt: Maus Monoclonal TU-30 unconjugated
Produktnummer ABIN94313
  • Target Alle TUBG1 Antikörper anzeigen
    TUBG1 (Tubulin, gamma 1 (TUBG1))
    Bindungsspezifität
    • 13
    • 9
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 64
    • 30
    • 26
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Ratte, Maus, Huhn, Rind (Kuh), Pflanzen, Protozoa, Schwein
    Wirt
    • 48
    • 19
    Maus
    Klonalität
    • 44
    • 23
    Monoklonal
    Konjugat
    • 42
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser TUBG1 Antikörper ist unkonjugiert
    Applikation
    • 49
    • 22
    • 12
    • 11
    • 11
    • 9
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Spezifität
    The antibody TU-30 recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope was located in the amino acid sequence TRPDYI (aa439-444 in human), which is present on human gamma-tubulin 1 but not on human gamma-tubulin 2.
    Kreuzreaktivität (Details)
    Human, Porcine, Mouse, Rat, Bovine, Chicken, Protozoa, Plants
    Aufreinigung
    Purified by protein-A affinity chromatography.
    Reinheit
    > 95 % (by SDS-PAGE)
    Immunogen
    C-terminal peptide of gamma-tubulin counjugated to KLH.
    Klon
    TU-30
    Isotyp
    IgG1
  • Applikationshinweise
    Immunocytochemistry: Recommended dilution: 1-2 μg/mL. Staining technique: (a) Fix cells for 10 min in methanol at -20 °C and for 6 min in acetone at -20 °C, (b) Fix cells directly in methanol for 10 min at -20 °C or in acetone for 10 min at -20 °C. Positive control: P-19 murine embryonal carcinoma cell line, 3T3 murine fibroblasts. The antibody TU-30 stains only fixed cells.
    Western blotting: Recommended dilution 1-2 μg/mL, reducing conditions.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Do not freeze.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8°C. Do not freeze.
  • Vulprecht, David, Tibelius, Castiel, Konotop, Liu, Bestvater, Raab, Zentgraf, Izraeli, Krämer: "STIL is required for centriole duplication in human cells." in: Journal of cell science, (2012) (PubMed).

    Löffler, Fechter, Matuszewska, Saffrich, Mistrik, Marhold, Hornung, Westermann, Bartek, Krämer: "Cep63 recruits Cdk1 to the centrosome: implications for regulation of mitotic entry, centrosome amplification, and genome maintenance." in: Cancer research, Vol. 71, Issue 6, pp. 2129-39, (2011) (PubMed).

    Koledova, Kafkova, Kraemer, Divoky: "DNA damage-induced degradation of Cdc25A does not lead to inhibition of Cdk2 activity in mouse embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 3, pp. 450-61, (2010) (PubMed).

    Katsetos, Reddy, Dráberová, Smejkalová, Del Valle, Ashraf, Tadevosyan, Yelin, Maraziotis, Mishra, Mörk, Legido, Nissanov, Baas, de Chadarévian, Dráber: "Altered cellular distribution and subcellular sorting of gamma-tubulin in diffuse astrocytic gliomas and human glioblastoma cell lines." in: Journal of neuropathology and experimental neurology, Vol. 65, Issue 5, pp. 465-77, (2006) (PubMed).

    Sulimenko, Dráberová, Sulimenko, Macurek, Richterová, Dráber, Dráber: "Regulation of microtubule formation in activated mast cells by complexes of gamma-tubulin with Fyn and Syk kinases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 12, pp. 7243-53, (2006) (PubMed).

    Libusová, Sulimenko, Sulimenko, Hozák, Dráber: "gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms." in: Experimental cell research, Vol. 295, Issue 2, pp. 375-86, (2004) (PubMed).

    Binarová, Cenklová, Hause, Kubátová, Lysák, Dolezel, Bögre, Dráber: "Nuclear gamma-tubulin during acentriolar plant mitosis." in: The Plant cell, Vol. 12, Issue 3, pp. 433-42, (2000) (PubMed).

    Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, (1996) (PubMed).

  • Target
    TUBG1 (Tubulin, gamma 1 (TUBG1))
    Andere Bezeichnung
    gamma-tubulin 1 (TUBG1 Produkte)
    Synonyme
    GCP-1 antikoerper, TUBG antikoerper, TUBGCP1 antikoerper, TUBC antikoerper, TUBG3 antikoerper, gamma-tubulin antikoerper, Tubg2 antikoerper, xgam antikoerper, 1500010O08Rik antikoerper, AI451582 antikoerper, AI503389 antikoerper, Tubg antikoerper, tubgl antikoerper, zgc:55894 antikoerper, tubulin gamma 1 antikoerper, gamma-tubulin 1 antikoerper, gamma-tubulin antikoerper, tubulin gamma 1 L homeolog antikoerper, Tubulin gamma chain antikoerper, tubulin, gamma 1 antikoerper, tubulin gamma-1 chain antikoerper, TUBG1 antikoerper, tubg1 antikoerper, tubg1.L antikoerper, tbg-1 antikoerper, Tubg1 antikoerper, LOC101686824 antikoerper, LOC101103906 antikoerper
    Hintergrund
    Tubulin gamma 1,The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01 % of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface. Humans possess two gamma-tubulin genes. Gamma-tubulin 1 represents a ubiquitous isotype, whereas gamma-tubulin 2 is found predominantly in the brain, where it may be endowed with divergent functions beyond microtubule nucleation.,TUBG
    Gen-ID
    7283
    UniProt
    P23258
    Pathways
    Microtubule Dynamics, M Phase
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