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Lhcb4 Antikörper

Lhcb4 Reaktivität: Arabidopsis thaliana, Green bean, Oryza sativa, Pisum sativum, Mais (Zea mays) WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN5684016
  • Target
    Lhcb4 (Cp29 Chlorophyll A/B Binding Protein of Plant Psii (Lhcb4))
    Reaktivität
    Arabidopsis thaliana, Green bean, Oryza sativa, Pisum sativum, Mais (Zea mays)
    Wirt
    Kaninchen
    Klonalität
    Polyklonal
    Applikation
    Western Blotting (WB)
    Spezifität
    The antibody is very specific for salmonid inducible form - hsp70.
    Kreuzreaktivität (Details)
    No cross-reactivity with: Chlamydomonas reinhardtii
    Produktmerkmale
    Expected / apparent Molecular Weight of the Antigene: 31.9 / 29 kDa for Arabidopsis thaliana
    Aufreinigung
    affinity purified
    Immunogen
    BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb4 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana (Lhcb4.1 At5g01530 and Lhcb4.2 At5g08940).
    Isotyp
    IgG
  • Applikationshinweise
    Recommended Dilution: 1 : 2000 - 1 : 5000 with standard ECL (WB).
    Kommentare

    for image of detection please refer to Kusaba et al. (2007)

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    For reconstitution add 200 µL of sterile water
    Buffer
    PBS pH 7.4
    Handhabung
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Lagerung
    -20 °C
  • Yamatani, Sato, Masuda, Kato, Morita, Fukunaga, Nagamura, Nishimura, Sakamoto, Tanaka, Kusaba: "NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll - protein complexes during leaf senescence." in: The Plant journal : for cell and molecular biology, Vol. 74, Issue 4, pp. 652-62, (2013) (PubMed).

  • Target
    Lhcb4 (Cp29 Chlorophyll A/B Binding Protein of Plant Psii (Lhcb4))
    Hintergrund
    AGI Code: At5g01530
    Lhcb4 (CP29) is one of the 3 minor chlrorophyll a/b-binding proteins associated with Photosystem II in plants and algae. Lhcb4 has been suggested to act in the regulation of the chl a excited state concentration of PSII because of its ability of sensing lumenal pH resulting in reversible phosphorylation. In Arabidopsis thaliana 2 genes code for two isoforms Lhcb4.1 and Lhcb4.2. A third isoform (Lhcb4.3, At5g40100), probably only present in dicots, has found to be differently regulated and therefore has been denoted as Lhcb8.
    Molekulargewicht
    expected: 31.9 kDa, apparent: 29 kDa for Arabidopsis thaliana
    UniProt
    Q9XF88, Q07473
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