DNAJA3 Antikörper (AA 101-200) (Alexa Fluor 647)

Produktnummer ABIN917710

Produktdetails anti-DNAJA3 Antikörper (Alexa Fluor 647)

Target: DNAJA3 (DnaJ (Hsp40) Homolog, Subfamily A, Member 3 (DNAJA3))

Bindungsspezifität: AA 101-200

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DNAJA3 Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Homologie: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TID1/HCA57

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu DNAJA3

Target: DNAJA3 (DnaJ (Hsp40) Homolog, Subfamily A, Member 3 (DNAJA3))

Andere Bezeichnung: HCA57 (DNAJA3 Produkte)

Synonyme: Tid-1 antikoerper, 1200003J13Rik antikoerper, 1810053A11Rik antikoerper, C81173 antikoerper, Tid1l antikoerper, HCA57 antikoerper, TID1 antikoerper, hTID-1 antikoerper, DnaJ heat shock protein family (Hsp40) member A3 antikoerper, Dnaja3 antikoerper, DNAJA3 antikoerper

Hintergrund:

Synonyms: DnaJ Hsp40 homolog subfamily A member 3, DnaJ homolog subfamily A member 3 mitochondrial precursor, DnaJ protein Tid 1, DnaJ protein Tid1, HCA57, Hepatocellular carcinoma associated antigen 57, Highly similar to HYPOTHETICAL 105.9 KD PROTEIN F22B7.5 IN CHROMOSOME, hTid 1, hTid1, III [Caenorhabditis elegans], TID1, Tumorous imaginal discs Drosophila homolog, Tumorous imaginal discs protein Tid56 homolog, DNJA3_HUMAN.

Background: TID1 is a human homolog of the Drosophila tumor suppressor lethal tumerous imaginal discs and encodes two mitochondrial matrix localized splice variants of human Tid1 designated hTid1S and hTid1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperons for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid1L increases apoptosis induced by DNA damaging agents as mitomycin C and TNF alpha. A J domain mutant of hTid1L can dominantly suppress apoptosis and in sharp contrast the J domain mutant of hTid1S increases apoptosis. Expression of hTid1S and hTid1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti and pro apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.

Gen-ID: 9093

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity