PKC beta Antikörper (Protein Kinase C, beta) (pThr641)

Details for Product anti-PRKCB Antibody No. ABIN871421, Anbieter: Anmelden zum Anzeigen
Antigen
  • PKC-beta
  • PKCB
  • PRKCB1
  • PRKCB2
  • A130082F03Rik
  • PKC-Beta
  • Pkcb
  • Prkcb1
  • Prkcb2
  • PKC-B
  • Prkcb
  • prkcb1
  • zgc:63591
  • PKC
  • PRKCB_tv2
  • protein kinase C beta
  • protein kinase C, beta
  • protein kinase C, beta b
  • PRKCB
  • Prkcb
  • prkcbb
Epitop
pThr641
30
16
15
15
13
9
9
8
8
5
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
167
117
114
20
20
14
10
5
4
2
2
2
2
2
1
1
1
1
Wirt
Kaninchen
183
18
8
5
Klonalität
Polyklonal
Konjugat
Dieser PKC beta Antikörper ist unkonjugiert
16
14
6
5
5
5
2
2
2
1
Applikation
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
195
102
93
24
22
13
12
11
8
7
4
4
3
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Peptide sequence around phosphorylation site of pThr641 (E-L-T (p) -P-T) derived from Human PKCb Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Spezifität The antibody detects endogenous levels of PKCb only when phosphorylated at threonine 641.
Reinigung Affinity purified
Plasmids, Primers & others Plasmide, Primers & weitere PKC beta products on genomics-online (e.g. as negative or positive controls)
Andere Bezeichnung PKCbeta (PRKCB Antibody Abstract)
Hintergrund Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
Molekulargewicht 82 kDa
Gen-ID 5579
NCBI Accession NP_002729
UniProt P05771
Pathways WNT Signalweg, T-Zell Rezeptor Signalweg, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded protein response
Applikations-hinweise Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Produkt verwendet in: Losso, Truax, Richard: "trans-resveratrol inhibits hyperglycemia-induced inflammation and connexin downregulation in retinal pigment epithelial cells." in: Journal of agricultural and food chemistry, Vol. 58, Issue 14, pp. 8246-52, 2010 (PubMed).

Allgemeine Veröffentlichungen Zhang, Liao, Dufau et al.: "Phosphatidylinositol 3-kinase/protein kinase Czeta-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated derepression [corrected] of ..." in: Molecular and cellular biology, Vol. 26, Issue 18, pp. 6748-61, 2006 (PubMed).

Castoria, Migliaccio, Di Domenico, Lombardi, de Falco, Varricchio, Bilancio, Barone, Auricchio: "Role of atypical protein kinase C in estradiol-triggered G1/S progression of MCF-7 cells." in: Molecular and cellular biology, Vol. 24, Issue 17, pp. 7643-53, 2004 (PubMed).

Marcil, Harbour, Houle, Naccache, Bourgoin: "Monosodium urate-crystal-stimulated phospholipase D in human neutrophils." in: The Biochemical journal, Vol. 337 ( Pt 2), pp. 185-92, 1999 (PubMed).

Bornancin, Parker: "Phosphorylation of threonine 638 critically controls the dephosphorylation and inactivation of protein kinase Calpha." in: Current biology : CB, Vol. 6, Issue 9, pp. 1114-23, 1997 (PubMed).

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