CDC25B Antikörper (pSer149)

Produktnummer ABIN871399

Produktdetails anti-CDC25B Antikörper

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Bindungsspezifität: pSer149

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDC25B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: The antibody detects endogenous level of Cdc25B protein only when phosphorylated at serine 149.

Aufreinigung: Affinity purified

Immunogen: Peptide sequence around phosphorylation site of pSer149 (F-R-S (p) -L-P) derived from Mouse Cdc25B. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-CDC25B Antikörper (ABIN871399)

Xiao, Liu, Hou, Cui, Wu, Fan, Sun, Meng, Yang, Wang, Yu: "Ser149 is another potential PKA phosphorylation target of Cdc25B in G2/M transition of fertilized mouse eggs." in: The Journal of biological chemistry, Vol. 286, Issue 12, pp. 10356-66, (2011) (PubMed).

Details zu CDC25B

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Andere Bezeichnung: Cdc25B (CDC25B Produkte)

Synonyme: CDC25B antikoerper, AI604853 antikoerper, cell division cycle 25B antikoerper, cdc25b antikoerper, CDC25B antikoerper, Cdc25b antikoerper

Hintergrund: Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Molekulargewicht: 62 kDa

Gen-ID: 12531

NCBI Accession: NP_001104

UniProt: Q9DBN8

Pathways: Zellzyklus, M Phase, Autophagie