CD200 Antikörper (AA 41-140)

Produktnummer ABIN761396

Produktdetails anti-CD200 Antikörper

Target: CD200

Bindungsspezifität: AA 41-140

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD200 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Human

Homologie: Mouse,Rat

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD200

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IHC)

Validierung #101517 (Immunohistochemistry)

by: Department of Pathology and Molecular Medicine, McMaster University

No.: #101517

Datum: 11.07.2017

Antigen: CD200

Chargennummer: AG06145442

Validierte Anwendung: Immunohistochemistry

Positivkontrolle: Human 7 week normal karyotype elective termination placenta

Negativkontrolle: Placental villus from 7w gestation karyotype-normal pregnancy, stained with KLH antibody ABIN401183

Bewertung:

Passed. ABIN761396 specifically labels the CD200 in human placental tissue.

Validierungsbilder

Staining with ABIN761396 at dilutions 1:200, 1:250, 1:300, and 1:400 (A, B, C, and D) using a 7 week elective termination of a pregnancy that tested negative for trisomies (Clark et al. (2017) Am. J. Reprod. Immunol). Optimal staining was obtained at 1:200 dilution. Anti-KLH control (E) at 1:200 was negative.

Protokoll

Primärantikörper: ABIN761396

Sekundärantikörper: Bond Polymer Refine Detection kit (Leica, DS9800, lot 49232)

Full Protocol:

• Fix human placental tissue in 10% buffered formalin for 24h at RT.
• Process and embed tissue in paraffin.
• Cut paraffin blocks with a Leica CM2255 Microtome into 4µm sections.
• Affix sections to positively charged slides and air dry ON at RT.
• Deway and hydrate the slides on an automated Leica BOND Rx stainer.
• Antigen retrieval on the Leica BOND Rx automated stainer using epitope retrieval buffer 2 (Leica, AR9640, lot ER20172).
• Stain slides with primary
• • Rabbit anti-CD200 antibody (AA 45-95) antibody (antibodies-online, ABIN761396, lot 980502W),
  • rabbit anti-CD200 Receptor 1-Like (CD200R1L) (AA 150-200) antibody(antibodies-online, ABIN1715098, lot 9A13M60), or
  • rabbit anti-KLH antibody (antibodies-online, ABIN401183, lot 304770)
• diluted 1:200 in Power Vision IHC Super Blocker (Leica, PV6122). The staining protocol incorporates a modified Leica standard protocol IHC-F (which omits the post-primary step) and uses the standard times outlined in the machine protocol.
• Stain sections with Bond Polymer Refine Detection kit (Leica, DS9800, lot 49232) containing peroxidase block, post primary antibody, polymer as well as DAB chromogen and hematoxylin counterstain for times outlined in the standard protocol IHC-F.
• Remove slides from the Leica Bond Rx and then dehydrate in ethanol and clear in xylene.
• Mount slides in permount mounting medium (Fisher Scientific, SP15-500, lot 162767).
• After the slide-coverslip edges are dry, scan slides using Imagescope and photograph at 400x.

Anmerkungen:

• Several dilutions from 1:200 to 1:400 of ABIN761396 were tested and 1:200 was found to be optimal.

• The staining pattern of ABIN761396 matches our results with the rabbit anti-CD200 polyclonal antibody RB846 and observations made by others. It is also consistent with staining with the CD200R1L antibody ABIN1715098).

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-CD200 Antikörper (ABIN761396)

Clark, Arredondo, Dhesy-Thind: "The CD200 tolerance-signaling molecule and its receptor, CD200R1, are expressed in human placental villus trophoblast and in peri-implant decidua by 5 weeks' gestation." in: Journal of reproductive immunology, Vol. 112, pp. 20-3, (2015) (PubMed).

Details zu CD200

Target: CD200

Andere Bezeichnung: Cd200 (CD200 Produkte)

Synonyme: MOX1 antikoerper, MOX2 antikoerper, MRC antikoerper, OX-2 antikoerper, CD200 antikoerper, Cspmo2 antikoerper, MRCOX2 antikoerper, Mox2 antikoerper, DKFZp459C0716 antikoerper, OX2 antikoerper, CD200 molecule antikoerper, Cd200 molecule antikoerper, CD200 antigen antikoerper, CD200 antikoerper, Cd200 antikoerper

Hintergrund:

Synonyms: MRC, MOX1, MOX2, OX-2, OX-2 membrane glycoprotein, CD200, My033

Background: Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.

Gen-ID: 4345

UniProt: P41217