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AKT 1/2/3 Antikörper (pSer472)

Reaktivität: Human, Maus, Ratte WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN756457
  • Target Alle AKT 1/2/3 Produkte
    AKT 1/2/3
    Bindungsspezifität
    • 25
    • 23
    • 23
    • 23
    • 20
    • 20
    • 18
    • 17
    • 16
    • 16
    • 16
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer472
    Reaktivität
    • 164
    • 152
    • 138
    • 46
    • 31
    • 28
    • 25
    • 15
    • 9
    • 6
    • 4
    • 4
    Human, Maus, Ratte
    Wirt
    • 162
    • 2
    Kaninchen
    Klonalität
    • 151
    • 13
    Polyklonal
    Konjugat
    • 41
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser AKT 1/2/3 Antikörper ist unkonjugiert
    Applikation
    • 147
    • 92
    • 91
    • 90
    • 35
    • 31
    • 29
    • 20
    • 9
    • 7
    • 6
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Spezifität
    This antibody will cross react with Akt1 when phosphorylated at Ser473 and Akt2 when phosphorylated at Ser474.
    Kreuzreaktivität
    Human, Maus, Ratte
    Homologie
    Dog,Cow,Sheep,Pig,Chicken,Rabbit
    Aufreinigung
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic phosphopeptide derived from human AKT3 around the phosphorylation site of Ser472
    Isotyp
    IgG
  • Applikationshinweise
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Wang, Zhang, Liu, Yang, Zou, Zhou, Miao, Chen, Xiong, Wen: "Neat1-miRNA204-5p-PI3K-AKT axis as a potential mechanism for photodynamic therapy treated colitis in mice." in: Photodiagnosis and photodynamic therapy, Vol. 24, pp. 349-357, (2019) (PubMed).

  • Target
    AKT 1/2/3
    Andere Bezeichnung
    AKT1/2/3 (AKT 1/2/3 Produkte)
    Hintergrund

    Synonyms: MPPH, PKBG, MPPH2, PRKBG, STK-2, PKB-GAMMA, RAC-gamma, RAC-PK-gamma, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, AKT3

    Background: AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.

    Gen-ID
    10000
    UniProt
    Q9Y243
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