Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

PI3K p85 alpha/gamma Antikörper (pTyr199, pTyr467)

PI3K p85a/g Reaktivität: Human, Maus, Ratte, Kaninchen WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN744743
  • Target Alle PI3K p85 alpha/gamma (PI3K p85a/g) Produkte
    PI3K p85 alpha/gamma (PI3K p85a/g) (Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g))
    Bindungsspezifität
    • 17
    • 17
    • 1
    • 1
    • 1
    pTyr199, pTyr467
    Reaktivität
    • 21
    • 21
    • 21
    • 15
    • 5
    • 1
    Human, Maus, Ratte, Kaninchen
    Wirt
    • 21
    Kaninchen
    Klonalität
    • 21
    Polyklonal
    Konjugat
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser PI3K p85 alpha/gamma Antikörper ist unkonjugiert
    Applikation
    • 21
    • 13
    • 12
    • 12
    • 8
    • 6
    • 4
    • 3
    • 2
    • 2
    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Kreuzreaktivität
    Human, Maus, Kaninchen, Ratte
    Homologie
    Dog,Cow,Pig,Horse
    Aufreinigung
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic phosphopeptide derived from human PI3K around the phosphorylation site of p85 alphaTyr467
    Isotyp
    IgG
  • Applikationshinweise
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #029801 (Western Blotting)
    'Independent Validation' Siegel
    by
    Alamo Laboratories Inc
    No.
    #029801
    Datum
    26.08.2014
    Antigen
    Chargennummer
    120522
    Validierte Anwendung
    Western Blotting
    Positivkontrolle
    HeLa G2/M Phase cells
    Negativkontrolle
    C6/36 cells (non-reactive species)
    Bewertung
    A strong band was observed in the positive control sample at the correct molecular weight, this band is absent from the negative control. Several bands were observed in the negative control sample which may represent non-specific binding.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antigen: PI3K p85 /p55 (pTyr467)
    • Catalog number: ABIN744743
    • Lot number: 120522
    • Antibody Dilution: 1:200
    Sekundärantikörper
    • Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
    • Lot number: L170-6515
    • Antibody Dilution: 1:10,000
    Full Protocol
    • 1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
    • 2. 18 μl of heated culture-media were loaded and resolved on 8-16% SDS-polyacrylamide gel.
    • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
    • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
    • 5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
    • 6. The membrane was rinsed with TBST once.
    • 7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 10 hours at 4°C.
    • 8. The membrane was rinsed in TBST thrice for 5 minutes each.
    • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
    • 10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
    • 11. The membrane was rinsed in TBS twice for 30 seconds each.
    • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 15 minutes.
    • 13. The membrane was rinsed three times TBST.
    • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
    • 15. The membrane was washed in TBST 2 times for 10 minutes each.
    • 16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.
    Anmerkungen
    - No experimental challenges noted.
  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Jeong, Liu, Kim: "Dried plum and chokeberry ameliorate d-galactose-induced aging in mice by regulation of Pl3k/Akt-mediated Nrf2 and Nf-kB pathways." in: Experimental gerontology, Vol. 95, pp. 16-25, (2017) (PubMed).

    Padiya, Chowdhury, Borkar, Srinivas, Pal Bhadra, Banerjee: "Garlic attenuates cardiac oxidative stress via activation of PI3K/AKT/Nrf2-Keap1 pathway in fructose-fed diabetic rat." in: PLoS ONE, Vol. 9, Issue 5, pp. e94228, (2014) (PubMed).

    Sun, Yang, Luo, Wang, Chen, Zhang, Wang, Li: "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." in: Theriogenology, (2014) (PubMed).

  • Target
    PI3K p85 alpha/gamma (PI3K p85a/g) (Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g))
    Andere Bezeichnung
    PI3 kinase p85 alpha + gamma + (PI3K p85a/g Produkte)
    Hintergrund

    Synonyms: p85, AGM7, GRB1, IMD36, p85-ALPHA, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PIK3R1

    Background: Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p11 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.

    Gen-ID
    5295
    UniProt
    P27986
Sie sind hier:
Kundenservice