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GDNF Antikörper (AA 121-211)

GDNF Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN736536
  • Target Alle GDNF Antikörper anzeigen
    GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
    Bindungsspezifität
    • 15
    • 9
    • 8
    • 6
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 121-211
    Reaktivität
    • 79
    • 42
    • 31
    • 15
    • 15
    • 1
    Human, Maus, Ratte, Rind (Kuh), Kaninchen
    Wirt
    • 74
    • 6
    • 4
    • 3
    Kaninchen
    Klonalität
    • 73
    • 13
    • 1
    Polyklonal
    Konjugat
    • 39
    • 10
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser GDNF Antikörper ist unkonjugiert
    Applikation
    • 77
    • 32
    • 25
    • 18
    • 18
    • 13
    • 12
    • 12
    • 6
    • 6
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Kreuzreaktivität
    Rind (Kuh), Human, Maus, Kaninchen, Ratte
    Homologie
    Dog,Cow,Pig,Horse,Chicken
    Aufreinigung
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human GDNF
    Isotyp
    IgG
  • Applikationshinweise
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #102003 (Immunofluorescence)
    'Independent Validation' Siegel
    by
    Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
    No.
    #102003
    Datum
    12.02.2018
    Antigen
    GDNF
    Chargennummer
    9F01M8
    Validierte Anwendung
    Immunofluorescence
    Positivkontrolle
    mouse hippocampus and putamen
    Negativkontrolle
    no primary control
    Bewertung

    ABIN736536 works in IF albeit with some background staining that we have been unable to eliminate although using different dilutions to increase the signal-to-noise ratio.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN736536
    Sekundärantikörper
    anti-rabbit AF488 conjugated antibody (Life Technologies)
    Full Protocol
    • Perfuse mouse with 4% paraformaldehyde in 0.1M phosphate buffer (PB) pH7.4.
    • Post-fix spinal cord and brain blocks in the same fixative for additional 2h at RT.
    • Wash spinal cord and brain blocks several times with PBS.
    • Cut blocks with a vibratome (Leica, VT1000 S) into 70µm thick transverse sections.
    • Cut dorsal root ganglions (DRGs) with a cryostat into 17µm thick sections after cryoprotection and glass mounting.
    • Block free floating vibratome and glass mounted cryostat sections with blocking solution (0.01M PBS5% Normal Goat Serum (NGS; Sigma, G9023, lot SLBV1396), 0.1% Triton X-100 (BioRad, 161-0407, lot 00583) for 1h at RT.
    • Incubate sections with primary rabbit anti-GDNF antibody (antibodies-online, ABIN736536, lot 9F01M8) diluted 1:100 in blocking solution ON at RT.
    • Wash sections 4x for 5min with 0.01M PBS.
    • Incubate sections with secondary goat anti-rabbit AF488 conjugated antibody (Life Technologies) diluted 1:500 in PBS for 1h at RT.
    • Wash sections 4x for 5min with 0.01M PBS.
    • Mount sections in Fluoroshield (Sigma, F6182, lot MKCB0153V).
    Anmerkungen
    • Different dilutions were also tested (1:200, 1:500, 1:2000) with or without Triton-X but the 1:100 dilution and the use of Triton-X in the blocking solution gave the best results.

    • Staining is mainly present in the cytoplasm of small-size neurons in the DRG, consistent with what has been previously described. ABIN736536 stains fibers in superficial laminae of the dorsal horn of the spinal cord. In the hippocampus staining is present in the CA1 both in the pyramidal neurons (cell body and dendrites) and in individual interneurons. In the putamen ABIN736536 stains neuron cell bodies and some dendrites.

  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Zhang, Cai, Song, Dong, Hou, Lv: "Normalization of ventral tegmental area structure following acupuncture in a rat model of heroin relapse." in: Neural regeneration research, Vol. 9, Issue 3, pp. 301-7, (2014) (PubMed).

  • Target
    GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
    Andere Bezeichnung
    GDNF (GDNF Produkte)
    Synonyme
    gdnf antikoerper, GDNF antikoerper, AI385739 antikoerper, gdnfa antikoerper, hfb1-gdnf antikoerper, ATF1 antikoerper, ATF2 antikoerper, HFB1-GDNF antikoerper, HSCR3 antikoerper, glial cell derived neurotrophic factor antikoerper, glial cell derived neurotrophic factor a antikoerper, glial cell line derived neurotrophic factor antikoerper, glial cell derived neurotrophic factor L homeolog antikoerper, Gdnf antikoerper, gdnfa antikoerper, GDNF antikoerper, gdnf.L antikoerper
    Hintergrund

    Synonyms: ATF1, ATF2, HSCR3, HFB1-GDNF, Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF

    Background: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

    Gen-ID
    2668
    UniProt
    P39905
    Pathways
    RTK Signalweg, Synaptic Membrane, Tube Formation, Autophagie, Smooth Muscle Cell Migration
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