GDNF Antikörper (Glial Cell Line Derived Neurotrophic Factor) (AA 166-211)

Details for Product anti-GDNF Antibody No. ABIN736536, Anbieter: Anmelden zum Anzeigen
Antigen
  • ATF
  • BDPLT5
  • QPD
  • UPA
  • URK
  • u-PA
  • gdnf
  • GDNF
  • AI385739
  • gdnfa
  • hfb1-gdnf
  • ATF1
  • ATF2
  • HFB1-GDNF
  • HSCR3
  • plasminogen activator, urokinase
  • glial cell derived neurotrophic factor
  • glial cell derived neurotrophic factor a
  • glial cell line derived neurotrophic factor
  • glial cell derived neurotrophic factor L homeolog
  • PLAU
  • Gdnf
  • gdnfa
  • GDNF
  • gdnf.L
Epitop
AA 166-211
16
14
9
9
6
6
4
3
2
2
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
164
77
56
1
Wirt
Kaninchen
151
22
9
4
Klonalität
Polyklonal
Konjugat
Dieser GDNF Antikörper ist unkonjugiert
18
11
7
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
172
47
37
28
27
21
13
12
10
6
5
4
4
2
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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'Independent Validation' Siegel
Antigen GDNF
Chargennummer 9F01M8
Validierte Anwendung Immunofluorescence
Positivkontrolle mouse hippocampus and putamen
Negativkontrolle no primary control
Bewertung

ABIN736536 works in IF albeit with some background staining that we have been unable to eliminate although using different dilutions to increase the signal-to-noise ratio.

Primärantikörper ABIN736536
Sekundärantikörper anti-rabbit AF488 conjugated antibody (Life Technologies)
Protokoll
  • Perfuse mouse with 4% paraformaldehyde in 0.1M phosphate buffer (PB) pH7.4.
  • Post-fix spinal cord and brain blocks in the same fixative for additional 2h at RT.
  • Wash spinal cord and brain blocks several times with PBS.
  • Cut blocks with a vibratome (Leica, VT1000 S) into 70µm thick transverse sections.
  • Cut dorsal root ganglions (DRGs) with a cryostat into 17µm thick sections after cryoprotection and glass mounting.
  • Block free floating vibratome and glass mounted cryostat sections with blocking solution (0.01M PBS5% Normal Goat Serum (NGS; Sigma, G9023, lot SLBV1396), 0.1% Triton X-100 (BioRad, 161-0407, lot 00583) for 1h at RT.
  • Incubate sections with primary rabbit anti-GDNF antibody (antibodies-online, ABIN736536, lot 9F01M8) diluted 1:100 in blocking solution ON at RT.
  • Wash sections 4x for 5min with 0.01M PBS.
  • Incubate sections with secondary goat anti-rabbit AF488 conjugated antibody (Life Technologies) diluted 1:500 in PBS for 1h at RT.
  • Wash sections 4x for 5min with 0.01M PBS.
  • Mount sections in Fluoroshield (Sigma, F6182, lot MKCB0153V).
Anmerkungen
  • Different dilutions were also tested (1:200, 1:500, 1:2000) with or without Triton-X but the 1:100 dilution and the use of Triton-X in the blocking solution gave the best results.

  • Staining is mainly present in the cytoplasm of small-size neurons in the DRG, consistent with what has been previously described. ABIN736536 stains fibers in superficial laminae of the dorsal horn of the spinal cord. In the hippocampus staining is present in the CA1 both in the pyramidal neurons (cell body and dendrites) and in individual interneurons. In the putamen ABIN736536 stains neuron cell bodies and some dendrites.

Validierungsbilder
Immunofluorescence validation image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536)

IF staining with ABIN736536 of mouse dorsal root ganglion (DRG, A), the dorsal hor...

Immunogen KLH conjugated synthetic peptide derived from human GDNF
Isotyp IgG
Reinigung Purified by Protein A.
Plasmids, Primers & others Plasmide, Primers & weitere GDNF products on genomics-online (e.g. as negative or positive controls)
Antigen Glial Cell Line Derived Neurotrophic Factor (GDNF)
Andere Bezeichnung GDNF (GDNF Antibody Abstract)
Hintergrund

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Subcellular location: Secreted

Synonyms: ATF1, ATF2, HSCR3, HFB1-GDNF, Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF

Gen-ID 2668
UniProt P39905
Pathways RTK Signalweg, Synaptic Membrane, Tube Formation, Autophagie, Smooth Muscle Cell Migration
Applikations-hinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Formalin-fixed and paraffin embedded rat brain labeled with Anti-GDNF Polyclonal Anti...
Immunohistochemistry (IHC) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-GDNF Polyc...
SDS-PAGE (SDS) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti GDNF Polyclonal ...
Western Blotting (WB) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Lane 1:U251 lysates and Lane 2: Mouse brain lysates probed with Rabbit Anti-GDNF Poly...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Formalin-fixed and paraffin embedded rat brain labeled with Anti-GDNF Polyclonal Anti...
Produkt verwendet in: Zhang, Cai, Song, Dong, Hou, Lv: "Normalization of ventral tegmental area structure following acupuncture in a rat model of heroin relapse." in: Neural regeneration research, Vol. 9, Issue 3, pp. 301-7, 2014 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Rat (Rattus)). Weitere Details: Sample: Brain

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