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Smad1/5 Antikörper (AA 361-465)

SMAD1/5 Reaktivität: Human IF (cc), IF (p), FACS, ELISA, IHC (fro), IHC (p), ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN732158
  • Target Alle Smad1/5 (SMAD1/5) Produkte
    Smad1/5 (SMAD1/5) (Smad1/5 Protein (SMAD1/5))
    Bindungsspezifität
    • 15
    • 15
    • 15
    AA 361-465
    Reaktivität
    • 16
    • 16
    • 16
    Human
    Wirt
    • 31
    Kaninchen
    Klonalität
    • 31
    Polyklonal
    Konjugat
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Dieser Smad1/5 Antikörper ist unkonjugiert
    Applikation
    • 25
    • 25
    • 16
    • 13
    • 5
    • 5
    • 5
    • 1
    Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    Kreuzreaktivität
    Human
    Homologie
    Mouse,Rat,Dog,Cow,Pig,Horse,Rabbit
    Aufreinigung
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human Smad 5
    Isotyp
    IgG
  • Applikationshinweise
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #029752 (Western Blotting)
    'Independent Validation' Siegel
    by
    Alamo Laboratories Inc
    No.
    #029752
    Datum
    03.07.2014
    Antigen
    Chargennummer
    120918
    Validierte Anwendung
    Western Blotting
    Positivkontrolle
    Hela cells
    Negativkontrolle
    c6/36 cells (non-reactive species)
    Bewertung
    A single major band was observed in the positive control at the correct molecular weight. Several additional faint bands appeared in both the positive and negative controls, but they did not indicate significant non-specific interaction.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antigen: SMAD, Mothers Against DPP Homolog 1 (SMAD1) (1:200 dilution)
    • Catalog number: ABIN732158
    • Lot number: 120918
    Sekundärantikörper
    • Antibody: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
    • Lot number: L170-6515
    Full Protocol
    • 1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
    • 2. 58 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
    • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
    • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
    • 5. The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 h.
    • 6. The membrane was rinsed with TBST once.
    • 7. The membrane was immersed with the protein side up in the primary antibody solution (anti-SMAD1; 1:200) in TBST containing 5% (W/V) BSA and incubated for 16 h at 4°C.
    • 8. The membrane was rinsed in TBST thrice for 5 min each.
    • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) BSA and incubated for 1 h at room temperature (~26°C) with gentle agitation.
    • 10. The membrane was rinsed in TBST thrice for 5 min each.
    • 11. The membrane was rinsed in TBS twice for 30 s each.
    • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
    • 13. The membrane was rinsed three times TBST.
    • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
    • 15. The membrane was washed in TBST 2 times for 10 min each.
    • 16. Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
    Anmerkungen
    - No experimental challenges noted.
  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Qu, Zheng, Gan, Lian, He, Li, Yuan, Yang, Li, Ji, Yan, Xu, Guo: "MiR-199a-3p suppresses proliferation and invasion of prostate cancer cells by targeting Smad1." in: Oncotarget, Vol. 8, Issue 32, pp. 52465-52473, (2017) (PubMed).

    Wei, Wang, Zhan, Chen, Fang, Zhang, Zhang: "Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation." in: The Journal of cell biology, Vol. 216, Issue 5, pp. 1455-1471, (2017) (PubMed).

  • Target
    Smad1/5 (SMAD1/5) (Smad1/5 Protein (SMAD1/5))
    Andere Bezeichnung
    Smad1 + Smad5 (SMAD1/5 Produkte)
    Hintergrund

    Synonyms: Mothers against decapentaplegic homolog 5, SMAD5, Mothers against decapentaplegic homolog 1, SMAD1

    Background: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.

    Gen-ID
    4090, 4086
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