GABRB2 Antikörper (Intracellular)

Produktnummer ABIN7581873

Der Kaninchen Polyklonal anti-GABRB2 Antikörper (ABIN7581873) detektiert spezifisch GABRB2 in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für GABRB2 Antikörper (Intracellular) (ABIN7581873)

Target: GABRB2 (gamma-aminobutyric Acid (GABA) A Receptor, beta 2 (GABRB2))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GABRB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GABRB2 Antikörper

Bindungsspezifität: AA 405-419, Intracellular

Verwendungszweck: A Rabbit Polyclonal antibody to GABA(A) β2 Receptor

Sequenz: (C)KAGLPRHSFG RNALE

Spezifität: Intracellular, 2nd loop.

Homologie: Mouse,Human - identical

Produktmerkmale: Anti-GABA(A) β2 Receptor Antibody (ABIN7581873) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABA(A) Receptor Beta 2 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KAGLPRHSFGRNALE, corresponding to amino acid residues 405 - 419 of rat GABRB2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GABRB2

Target: GABRB2 (gamma-aminobutyric Acid (GABA) A Receptor, beta 2 (GABRB2))

Andere Bezeichnung: GABRB2

Hintergrund: Gamma-Aminobutyric Acid Receptor Subunit Beta-2, GABA(A) receptor subunit beta-2, GABA(A) Receptor Beta 2, GABRB2,GABA receptors have two main subtypes: GABA(A) and GABA(B) receptors based on their amino acid sequence and structure. The GABA(A) receptors are ionotropic receptors while the GABA(B) receptors are metabotropic G-protein coupled receptors1.GABA(A) receptors are ion channels opened when GABA or its selective agonist muscimol binds to the receptor. In contrast, when the competitive antagonists bicuculline or gabazine binds to the GABA(A) receptors, the GABA-evoked current is decreased. GABA(A) receptors are blocked with picrotoxin, a non-competitive blocker, that blocks the GABA(A) receptor pore. Benzodiazepines and barbiturates are positive allosteric modulators of GABA(A) receptors and can enhance the currents of these channels2-4.To date, 19 GABA(A) receptor subunits have been identified: α 1-6, β 1-3, γ1-3, δ, ε, θ, p and ? 1-34. GABA(A)receptors are heteropentameric complexes formed by combinations of different GABA(A) receptor subunits 4.The major isoform of the GABA(A) receptor in the brain is composed of two α1, two β2, and one γ2 subunits that are encoded by the GABRA1, GABRB2, and GABRG2 genes respectively, accounting for 43 % of the GABA receptors in the mammalian brain 5. It is then not surprising that GABA(A) Receptor Beta 2, has been associated with several neuropsychiatric disorders such as schizophrenia, bipolar disorder, epilepsy, autism spectrum disorder, Alzheimer's disease, frontotemporal dementia and depression 5.

Gen-ID: 25451

UniProt: P63138

Pathways: Sensory Perception of Sound, Synaptic Membrane