CHRNA2 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7886585

Der Kaninchen Polyklonal anti-CHRNA2 Antikörper (ABIN7886585) detektiert spezifisch CHRNA2 in WB, IHC, IC, IF und LCI. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für CHRNA2 Antikörper (Extracellular, N-Term) (ABIN7886585)

Target: CHRNA2 (Cholinergic Receptor, Nicotinic, alpha 2 (Neuronal) (CHRNA2))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHRNA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunochromatography (IC), Immunofluorescence (IF), Live Cell Imaging (LCI)

Produktdetails anti-CHRNA2 Antikörper

Bindungsspezifität: AA 189-201, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α2

Sequenz: (C)DLEQMERTVD LKD

Homologie: Mouse - identical, human - 12,13 amino acid residues identical

Produktmerkmale: Highly specific antibody directed against an extracellular epitope of rat nAChRα2. Anti-Nicotinic Acetylcholine Receptor α2 (extracellular) (CHRNA2) Antibody (ABIN7581852) can be used in western blot, immunohistochemistry and immunocytochemistry applications. The antibody is specially suited to recognize nAChRα2 in live cells. It has been designed to recognize nAChRα2 from rat mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)DLEQMERTVDLKD, corresponding to amino acid residues 189-201 of rat nAChRalpha2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200-1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu CHRNA2

Target: CHRNA2 (Cholinergic Receptor, Nicotinic, alpha 2 (Neuronal) (CHRNA2))

Andere Bezeichnung: CHRNA2

Hintergrund:

Synonyms: nAChR α2, Cholinergic receptor nicotinic alpha 2, Neuronal acetylcholine receptor subunit alpha-2

Description: Nicotinic acetylcholine receptors (nAChRs) mediate the physiological effects of exogenous nicotine. They also play critical physiological roles throughout the brain and body by mediating cholinergic excitatory neurotransmission, modulating the release of neurotransmitters, and have longer-term effects on gene expression and cellular connections1.nAChRs are pentameric complexes made up of combinations of a number of different nAChR subunits, which can be classified as α subunits, containing two cysteine residues at positions analogous to Cys192 and Cys193, and non-alpha subunits ('structural' subunits), which can be defined as β subunits when they are expressed in the vertebrate nervous system2. There are nine α subunits (α2-α10) and three β subunits (β2, β3, and β4) in the CNS3. Nicotinic receptors are assembled as combinations of α (2-6) and and β (2-4) subunits. All α subunits are expressed in neuronal cells except for the α1 subunit which is specifically expressed in skeletal muscle. They are also expressed in non-neuronal cells such as bronchial epithelial cells4, as well lymphocytes5.In humans, a mutant α2 subunit has been identified, which forms nAChRs with increased agonist sensitivity and causes a form of familial epilepsy6. Further, an α2 subunit null mouse model has been used to demonstrate a role for α2 nAChR in nicotine-induced modulation of long-term potentiation in the mouse hippocampal CA1 region, which may underlie some of the cognitive effects of nicotine7.

Gen-ID: 170945

UniProt: P12389