Rekombinanter mTagBFP Antikörper (DBCO)
Reaktivität: Entacmaea quadricolor
FACS, ICC, IF
Wirt: Alpaca
Monoclonal
1H7
DBCO
single-domain Antibody (sdAb)
Recombinant Antibody
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- Target
- mTagBFP
- Antikörpertyp
- Recombinant Antibody
- Fragment
- single-domain Antibody (sdAb)
- Reaktivität
- Entacmaea quadricolor
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Wirt
-
Alpaca
- Expressionssystem
- E.coli
-
Klonalität
- Monoklonal
-
Konjugat
- DBCO
- Applikation
- Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Spezifität
- Our sdAb binds strongly to TagRFP, Tag BFP and mTagBFP2, mTagBFP, mKate, mKate2, mTagRFP, mTagRFP657
- Aufreinigung
- Produced in: E.coli
- Immunogen
- Our sdAb binds strongly to TagRFP, Tag BFP and mTagBFP2.
- Klon
- 1H7
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-
- Applikationshinweise
- Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Rekonstitution
- Reconsitute with 250 µL 50% glycerol for storage at -80 °C
- Buffer
- sdAbs are lyophilized from PBS pH 7.4.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
-
- Target
- mTagBFP
- Hintergrund
- TagRFP was engineered in 2007 from TurboRFP (derived from Entacmaea quadricolor). Tag RFP displayed an extended fluorescence lifetime and showed a robust photostability. In 2008 after further mutagenesis and the exchange of ~12 amino acid residues, this red-emitting protein turned into a blue-emitting protein TagBFP. Only several years later, in 2011 it a monomeric version of TagBFP was discribed: mTagBFP2.
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