NEK2 Antikörper (AA 1-100)

Produktnummer ABIN713921

Produktdetails anti-NEK2 Antikörper

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Bindungsspezifität: AA 1-100

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Dog,Cow,Pig,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NEK2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-NEK2 Antikörper (ABIN713921)

Zeng, Han, Wang, Cai, Huang, Luo, Liu, Zhuo, Dai, Zhao, Liang, Zhong: "Overexpression of NIMA-related kinase 2 is associated with progression and poor prognosis of prostate cancer." in: BMC urology, Vol. 15, pp. 90, (2015) (PubMed).

Zhong, Guan, Dong, Yang, Liu, Zhang: "Examining Nek2 as a better proliferation marker in non-small cell lung cancer prognosis." in: Tumour biology, Vol. 35, Issue 7, pp. 7155-62, (2014) (PubMed).

Zhong, Guan, Liu, Zhang: "Aberrant expression of NEK2 and its clinical significance in non-small cell lung cancer." in: Oncology letters, Vol. 8, Issue 4, pp. 1470-1476, (2014) (PubMed).

Details zu NEK2

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Andere Bezeichnung: NEK2 (NEK2 Produkte)

Synonyme: AA617254 antikoerper, C77054 antikoerper, HsPK21 antikoerper, NEK2A antikoerper, NLK1 antikoerper, ATNEK2 antikoerper, NIMA-RELATED KINASE2 antikoerper, NIMA-related kinase 2 antikoerper, T9J14.24 antikoerper, T9J14_24 antikoerper, NIMA related kinase 2 antikoerper, NIMA (never in mitosis gene a)-related expressed kinase 2 antikoerper, NIMA-related kinase 2 antikoerper, NEK2 antikoerper, Nek2 antikoerper

Hintergrund:

Synonyms: NLK1, RP67, NEK2A, HsPK21, PPP1R111, Serine/threonine-protein kinase Nek2, HSPK 21, Never in mitosis A-related kinase 2, NimA-related protein kinase 2, NimA-like protein kinase 1, NEK2

Background: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP25 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC8. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC2 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC, SGOL1, NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.

Gen-ID: 4751

UniProt: P51955

Pathways: M Phase