RPA3 Antikörper

Produktnummer ABIN7118404

Produktdetails anti-RPA3 Antikörper

Target: RPA3 (Replication Protein A3, 14kDa (RPA3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RPA3 Antikörper ist unkonjugiert

Applikation: ELISA

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: replication protein A3, 14kDa

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Haltbarkeit: 12 months

Details zu RPA3

Target: RPA3 (Replication Protein A3, 14kDa (RPA3))

Andere Bezeichnung: RPA3 (RPA3 Produkte)

Synonyme: REPA3 antikoerper, 14kDa antikoerper, C330026P08Rik antikoerper, im:6908405 antikoerper, zgc:56310 antikoerper, zgc:86697 antikoerper, rpa3 antikoerper, MGC82610 antikoerper, RPA3 antikoerper, repa3 antikoerper, replication protein A3 antikoerper, replication protein A3 S homeolog antikoerper, replication protein A3 L homeolog antikoerper, RPA3 antikoerper, Rpa3 antikoerper, rpa3 antikoerper, rpa3.S antikoerper, rpa3.L antikoerper

Hintergrund: Synonyms:REPA3, RPA14 Background:As part of the heterotrimeric replication protein A complex(RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage(PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response(PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair(PubMed:7697716). Plays also a role in base excision repair(BER), probably through interaction with UNG(PubMed:9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA(PubMed:19010961). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C(RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange(PubMed:19996105).

Gen-ID: 6119

UniProt: P35244

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA