SCN3A Antikörper (Extracellular)

Produktnummer ABIN7043644

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch SCN3A in WB, IF, IC und LCI. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für SCN3A Antikörper (Extracellular) (ABIN7043644)

Target: SCN3A (Sodium Channel, Voltage-Gated, Type III, alpha Subunit (SCN3A))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SCN3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Produktdetails anti-SCN3A Antikörper

Bindungsspezifität: AA 277-288, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to SCN3A (NaV1.3) Channel

Spezifität: 3rd extracellular loop, domain I.

Kreuzreaktivität (Details): May recognize NaV1.1 and NaV1.2 due to a moderate homology between the chosen sequence and the sequences of NaV1.1 and NaV1.2 (although there are no five amino acids identical in a row as considered to be the minimum needed for antibody/antigen recognition).

Homologie: Mouse,human - 11,12 amino acid residues identical

Produktmerkmale: Anti-SCN3A (Nav1.3) (extracellular) Antibody is directed against an epitope of the rat NaV1.3 channel. Anti-SCN3A (NaV1.3) (extracellular) Antibody (ABIN7043644, ABIN7045250 and ABIN7045251) can be used in western blot, and live cell imaging applications. It has been designed to recognize NaV1.3 from rat, human and mouse samples. The antibody may recognize NaV1.1 and NaV1.2 due to a moderate homology between the chosen sequence and the sequences of NaV1.1 and NaV1.2 (although there are no five amino acids identical in a row as considered to be the minimum needed for antibody/antigen recognition).

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CSQWPPSDSAFE, corresponding to amino acid residues 277-288 of rat NaV1.3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IHC|FC

Negative Control: (ABIN7236847)

Blocking Peptide: (ABIN7236847)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu SCN3A

Target: SCN3A (Sodium Channel, Voltage-Gated, Type III, alpha Subunit (SCN3A))

Andere Bezeichnung: SCN3A

Hintergrund: BIII, Brain type III Na+ channel, Sodium channel protein type 3 subunit alpha,Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7 NaV1.3, also known as SCN3A, is highly expressed in embryonic sensory neurons and CNS, but its level dramatically decreases in adult rodents.8 Up-regulation of NaV1.3 channel expression was described in injured neurons and injured spinal cord.9-11

Alternative names: SCN3A (NaV1.3), BIII, Brain type III Na+ channel, Sodium channel protein type 3 subunit alpha

Gen-ID: 497770

NCBI Accession: NM_006922

UniProt: P08104