ASIC1 Antikörper (Extracellular)

Produktnummer ABIN7042913

Der Kaninchen Polyklonal anti-ASIC1 Antikörper wird verwendet zum Nachweis von ASIC1 in Proben von Ratte. Er wurde validiert für WB, IHC, IF, FACS, IC und LCI.

Kurzübersicht für ASIC1 Antikörper (Extracellular) (ABIN7042913)

Target: ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ASIC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunochromatography (IC), Live Cell Imaging (LCI)

Produktdetails anti-ASIC1 Antikörper

Bindungsspezifität: AA 368-382, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to ASIC Channels (ASIC1, ASIC2, ASIC3, ASIC4)

Spezifität: Extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: human - 12,rat - 12,rat,human - 14,ASIC1: Mouse,human - identicalASIC2: Mouse,15 amino acid residues identicalASIC3: Mouse,15 amino acid residues identicalASIC4: Mouse,15 amino acid residues identical, human - 11

Produktmerkmale: Anti-pan ASIC (extracellular) Antibody (ABIN7042913, ABIN7045258 and ABIN7045259) is directed against an epitope of rat ASIC1. The antibody recognizes ASIC1 through ASIC4. Anti-pan ASIC (extracellular) Antibody (ABIN7042913, ABIN7045258 and ABIN7045259) can be used in western blot, immunohistochemistry and immunocytochemistry in living cells. It has been designed to recognize all ASIC channels from rat, human and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)TRYGKELSMVKIPSK, corresponding to amino acid residues 368-382 of rat ASIC1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu ASIC1

Target: ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))

Andere Bezeichnung: ASIC

Hintergrund: Acid-sensing ion channel, Acid-sensing (proton-gated) ion channel, ACCN, ACCN2, ACCN3, ACCN4,ASIC channels belong to the ENaC/Degenerin superfamily and are proton-gated ion channels which are not voltage-dependent. Six different genes encode the different ASIC subunits and splice variants: ASIC1a, ASIC1b, ASIC2a, ASIC2b, ASIC3 and ASIC4.ASIC channels sense protons and interact with proteins (namely proteases) and other ions such as Zn2+ and Ca2+ through their extracellular domains. The extracellular loop of ASIC1a undergoes cleavage by extracellular trypsin (a serine protease) which is responsible for shifting the pH dependence of the channel activation and inactivation to more acidic pH 1.These channels are expressed in the brain and in the nervous system2 but have also been found in the retina, testes, and other tissues3,4 where they are involved in many physiological processes including nociception5, learning and memory6, mechanosensation7 among others.

Alternative names: ASIC, Acid-sensing ion channel, Acid-sensing (proton-gated) ion channel, ACCN, ACCN2, ACCN3, ACCN4

Gen-ID: 79123

UniProt: P55926