Histone H4 Antikörper (acLys12)

Produktnummer ABIN6972105

Produktdetails anti-Histone H4 Antikörper

Target: Histone H4

Bindungsspezifität: acLys12

Reaktivität: Human, Maus, Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC)

Produktmerkmale: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. The acetylation of histone H4 Lys12 is carried out by several histone acetyltransferases (HATs). Histone H4 Lys12 acetylation by TIP60 occurs in the nucleus, whereas Hat1-mediated acetylation of histone H4 Lys12 occurs in the cytoplasm, indicating the spatial regulation of histone acetylation. The acetylation of histone H4 Lys12 residue is commonly associated with transcriptional activation. Histone H4K12ac antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Dot blot, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Budding Yeast, Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.

Aufreinigung: Protein A Chromatography

Immunogen: This Histone H4 acetyl Lys12 antibody was raised against a peptide including acetyl-lysine 12 of histone H4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Details zu Histone H4

Target: Histone H4

Abstract: Histone H4 Produkte

Synonyme: hist1h4l antikoerper, H1ft antikoerper, Hist4 antikoerper, Hist4h4 antikoerper, H4 antikoerper, X04652 antikoerper, FO108 antikoerper, H4/n antikoerper, H4F2 antikoerper, H4FN antikoerper, HIST2H4 antikoerper, H4C13 antikoerper, H4C7 antikoerper, F8N16.2 antikoerper, F8N16_2 antikoerper, histone H4 antikoerper, BcDNA:RH52884 antikoerper, CG3379 antikoerper, Dmel\CG3379 antikoerper, H4r antikoerper, His4-88CD antikoerper, His4R antikoerper, histone cluster 1, H4a L homeolog antikoerper, histone cluster 1 H4 family member B antikoerper, histone cluster 2, H4 antikoerper, histone cluster 2 H4 family member a antikoerper, histone H4 antikoerper, Histone H4 replacement antikoerper, Histone H4 antikoerper, hist1h4a.L antikoerper, Hist1h4b antikoerper, Hist2h4 antikoerper, HIST2H4A antikoerper, H4 antikoerper, H4C14 antikoerper, HIS4 antikoerper, HIST2H4 antikoerper, His4r antikoerper, his-38 antikoerper

Molekulargewicht: 8 kDa

NCBI Accession: NP_778224