CD27 Antikörper (PE)
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- Target Alle CD27 Antikörper anzeigen
- CD27 (CD27 Molecule (CD27))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD27 Antikörper ist konjugiert mit PE
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Applikation
- Flow Cytometry (FACS)
- Kreuzreaktivität
- Pavian, Cynomolgus, Rhesusaffen
- Aufreinigung
- This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
- Klon
- O323
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product CD27 Primärantikörper
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- Applikationshinweise
- This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 μLper test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
- Kommentare
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5 uL (0.25 ug)/test
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- 2-8°C protected from light
- Haltbarkeit
- 12 months
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- Target
- CD27 (CD27 Molecule (CD27))
- Andere Bezeichnung
- CD27 (CD27 Produkte)
- Synonyme
- TNFRSF7 antikoerper, S152 antikoerper, Tnfrsf7 antikoerper, Tp55 antikoerper, S152. LPFS2 antikoerper, T14 antikoerper, CD27 molecule antikoerper, CD27 antigen antikoerper, CD27 antikoerper, Cd27 antikoerper
- Hintergrund
- The O323 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been in- cluded within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells.The O323 antibody may be used for analysis of CD27 expression on peripheral T cells, and is frequently used in combination with antibodies for IgD and IgM to distinguish naive vs. memory B cell populations. For iden- tification of Breg cells, this antibody has been used in combination with antibodies for CD25, CD27, CD1d, IL-10 and TGF-beta (Kessel et al. 2012. Autoimm. Rev. 11(9): 670-677). The antibody is also reported for cross-reactivity with Baboon, Cynomolgus and Rhesus CD27.
- Gen-ID
- 939
- UniProt
- P26842
- Pathways
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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