OGT Antikörper

Produktnummer ABIN6944354

Produktdetails anti-OGT Antikörper

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser OGT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: Recombinant protein within Human OGT aa 1000 to the C-terminus

Klon: 11F2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu OGT

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Andere Bezeichnung: OGT (OGT Produkte)

Synonyme: BcDNA:GH04245 antikoerper, CG10392 antikoerper, Dmel\\CG10392 antikoerper, OGT antikoerper, Ogt antikoerper, P1201 antikoerper, SXC antikoerper, Sxc antikoerper, l(2)02637 antikoerper, l(2)NC130 antikoerper, sxc/Ogt antikoerper, fm81g08 antikoerper, ogt antikoerper, wu:fc12b01 antikoerper, wu:fm81g08 antikoerper, HRNT1 antikoerper, O-GLCNAC antikoerper, 1110038P24Rik antikoerper, 4831420N21Rik antikoerper, AI115525 antikoerper, Ogtl antikoerper, O-linked N-acetylglucosamine (GlcNAc) transferase L homeolog antikoerper, super sex combs antikoerper, O-linked N-acetylglucosamine (GlcNAc) transferase, tandem duplicate 1 antikoerper, O-linked N-acetylglucosamine (GlcNAc) transferase antikoerper, O-linked GlcNAc transferase antikoerper, o-linked GlcNAc transferase antikoerper, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) antikoerper, ogt.L antikoerper, sxc antikoerper, ogt.1 antikoerper, OGT antikoerper, ogt antikoerper, GL50803_12081 antikoerper, GL50803_41701 antikoerper, THAPSDRAFT_264441 antikoerper, spyA antikoerper, Ogt antikoerper

Hintergrund:

Synonyms: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O-linked N-acetylglucosamine transferase 110 kDa subunit, OGT, OGT1, HRNT1, MRX106, HINCUT-1, O-GLCNAC.

Background: Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. O-GlcNAc transferase has been purified from rat liver. It exists as a heterotrimeric complex with two subunits of the same molecular mass and one shorter subunit. Both polypeptides are related, the short subunit band is either a proteolytic product of the polypeptide or the product of an alternative translation start site. O-GlcNAc transferase is expressed as multiple transcripts that are present in different amounts in various human tissues, with the highest levels of expression in pancreas. Immunofluorescence of human cells expressing rat O-GlcNAc transferase indicated that it is present in both the nucleus and cytosol. HeLa cells expressing O-GlcNAc transferase do not survive well during prolonged incubations, suggesting that this protein may be toxic to the cells.

Gen-ID: 8473

UniProt: O15294

Pathways: Regulation of Carbohydrate Metabolic Process