ABCC3 Antikörper (AA 815-957)

Produktnummer ABIN6940972

Der Maus Monoklonal anti-ABCC3 Antikörper (Klon ABCC3-2971) (ABIN6940972) detektiert spezifisch ABCC3 in IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für ABCC3 Antikörper (AA 815-957) (ABIN6940972)

Target: ABCC3 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 3 (ABCC3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ABCC3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: ABCC3-2971

Produktdetails anti-ABCC3 Antikörper

Bindungsspezifität: AA 815-957

Verwendungszweck: MRP3 (Multidrug Resistance-Associated Protein 3) Antibody

Spezifität: The two members of the large family of ABC transporters known to confer multidrug resistance in human cancer cells are the MDR1 P-glycoprotein and the multidrug-resistance protein MRP1. MRP1 is an integral membrane protein that contains an MDR-like core, an N-terminal membrane-bound region and a cytoplasmic linker, and it is expressed in various cerebral cells, as well as in lung, testis and peripheral blood. The MRP gene family also includes MRP2, which is alternatively designated cMOAT (for canalicular multispecific organic anion transporter) and MRP3, which are both conjugate export pumps exp- ressed predominantly in hepatocytes. MRP2 localizes exclusively to the apical membrane and is constitutively expressed at a high level in normal liver cells. Conversely, MRP3 localizes to the basolateral membrane where it also medi- ates the transport of the organic anion S-(2,4-dinitrophenyl-) glutathione toward the basolateral side of the membrane. MRP3 is normally expressed at comparatively lower levels than MRP2 and increases only when secretion across the apical membrane by MRP2 is impaired.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 815-957) of human MRP3 (ABCC3) protein (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Pancreas.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu ABCC3

Target: ABCC3 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 3 (ABCC3))

Andere Bezeichnung: ABCC3

Hintergrund:

ABC31, ATP-binding cassette sub-family C member 3 (ABCC3), Canalicular multispecific organic anion transporter 2 (CMOAT2), MLP2, MOATD, Multi-specific organic anion transporter D, Multidrug resistance-associated protein 3

Pancreas.

Molekulargewicht: 169/137/55/32/65kDa

Gen-ID: 8714, 463421

UniProt: O15438