BAX Antikörper (AA 3-16)

Produktnummer ABIN6940473

Produktdetails anti-BAX Antikörper

Target: BAX (BCL2-Associated X Protein (BAX))

Bindungsspezifität: AA 3-16

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BAX Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Spezifität: Recognizes a protein of 21 kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Aufreinigung: Purified by Protein A/G

Immunogen: A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.

Klon: SPM336

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Jurkat, K562, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin s lymphoma.

Known Application: Flow Cytometry (0.5-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Western Blot (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Andere Bezeichnung: BAX (BAX Produkte)

Synonyme: BAX-ALPHA antikoerper, bax-A antikoerper, xBax antikoerper, xlbax antikoerper, BAX antikoerper, bax antikoerper, fj16e01 antikoerper, wu:fc50b10 antikoerper, wu:fj16e01 antikoerper, BCL2L4 antikoerper, zgc:112983 antikoerper, BCL2 associated X, apoptosis regulator antikoerper, BCL2-associated X protein L homeolog antikoerper, BCL2-associated X protein antikoerper, bcl2-associated X protein, a antikoerper, bcl2-associated X protein, b antikoerper, BAX antikoerper, bax.L antikoerper, bax antikoerper, baxa antikoerper, Bax antikoerper, baxb antikoerper

Molekulargewicht: 21kDa

Gen-ID: 581

UniProt: Q07812

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response