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Rekombinanter Histone H1 Antikörper

Reaktivität: Human, Maus, Ratte IHC, StM Wirt: Kaninchen Monoclonal AE-4 unconjugated Recombinant Antibody
Produktnummer ABIN6939601
  • Target Alle Histone H1 Antikörper anzeigen
    Histone H1
    Antikörpertyp
    Recombinant Antibody
    Reaktivität
    • 106
    • 84
    • 77
    • 6
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 77
    • 28
    • 3
    • 2
    Kaninchen
    Klonalität
    • 70
    • 40
    Monoklonal
    Konjugat
    • 59
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Histone H1 Antikörper ist unkonjugiert
    Applikation
    • 51
    • 47
    • 46
    • 44
    • 20
    • 15
    • 13
    • 13
    • 8
    • 7
    • 6
    • 6
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Staining Methods (StM)
    Immunogen
    Nuclei of human leukemia biopsy cells
    Klon
    AE-4
    Isotyp
    IgG
    Top Product
    Discover our top product Histone H1 Primärantikörper
  • Applikationshinweise

    Positive Control: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma (IHC).

    Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-80 °C
    Informationen zur Lagerung
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Haltbarkeit
    24 months
  • Target
    Histone H1
    Andere Bezeichnung
    Histone H1 (Pan Nuclear Marker) (Histone H1 Produkte)
    Synonyme
    H10 antikoerper, H1FV antikoerper, H1 histone family member 0 antikoerper, H1F0 antikoerper
    Hintergrund
    Please note that this antibody is a recombinant Rabbit version of original anti-histone H1 antibody (Clone AE-4). Because the variable heavy (VH) and variable light (VL) domains are the same, recombinant antibody has the same exact reactivity as the original AE-4 MAb. There are several advantages of producing a recombinant version of a monoclonal antibody. For example, a recombinant antibody is a pure preparation of active immunoglobulin with no contaminating non-functional intact Ig or free light/heavy chains. Secondly, antibody can always be produced, even if hybridoma line is lost. Moreover, it adds the flexibility of converting the antibody to any species, isotype or format. Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. This antibody is extensively used as a pan-nuclear marker.
    Molekulargewicht
    ~30kDa
    Gen-ID
    3005
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