Laminin gamma 1 Antikörper
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- Target Alle Laminin gamma 1 (LAMC1) Antikörper anzeigen
- Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))
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Reaktivität
- Maus
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Wirt
- Ratte
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Klonalität
- Monoklonal
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Konjugat
- Dieser Laminin gamma 1 Antikörper ist unkonjugiert
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Applikation
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Spezifität
- Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This MAb reacts with laminin B2/1 chain of ~210 kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific MAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This MAb stains uniformly all human and murine basement membranes.
- Kreuzreaktivität (Details)
- Human. Mouse.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Murine EHS laminin preparation
- Klon
- A5
- Isotyp
- IgG2a kappa
- Top Product
- Discover our top product LAMC1 Primärantikörper
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- Applikationshinweise
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Normal colon or carcinomas.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
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- Target
- Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))
- Andere Bezeichnung
- LAMC1 (LAMC1 Produkte)
- Synonyme
- lamb2 antikoerper, Lamb2 antikoerper, B2e antikoerper, sly antikoerper, LAMB2 antikoerper, putative laminin gamma-1 chain antikoerper, laminin subunit gamma 1 antikoerper, laminin, gamma 1 antikoerper, Smp_163810 antikoerper, lamc1 antikoerper, Lamc1 antikoerper, LAMC1 antikoerper
- Hintergrund
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LAMB2, LAMC1, Laminin B2 chain, Laminin gamma 1, Laminin gamma 1 chain,Laminin, gamma 1 (LAMC1)
Cellular localisation: Basement membrane - Molekulargewicht
- 210kDa
- Gen-ID
- 3915, 609663
- UniProt
- P11047, Q5VYE7, Q6NVY8
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