C4A Antikörper (CF®488A)

Produktnummer ABIN6935975

Produktdetails anti-C4A Antikörper (CF®488A)

Target: C4A (C4a) (Complement Fragment C4a (C4a))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser C4A Antikörper ist konjugiert mit CF®488A

Applikation: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Coating (Coat), Staining Methods (StM)

Spezifität: This MAb is specific to Complement 4d (C4d) and reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 Molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human Complement 4d protein

Klon: C4D204

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Rejected Renal Transplant Tissue.

Known Application: ELISA (For coating use Ab at 1-2 μg/mL order Ab without BSA), Immunofluorescence (1:50-1:100),Immunohistochemistry (Formalin-fixed) (1:200-1:400 for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu C4A

Target: C4A (C4a) (Complement Fragment C4a (C4a))

Andere Bezeichnung: C4A (C4a Produkte)

Synonyme: C4 antikoerper, C4A2 antikoerper, C4A3 antikoerper, C4A4 antikoerper, C4A6 antikoerper, C4AD antikoerper, C4S antikoerper, CO4 antikoerper, CPAMD2 antikoerper, RG antikoerper, Slp antikoerper, C4-1 antikoerper, C4b antikoerper, complement C4A (Rodgers blood group) antikoerper, complement component 4A (Rodgers blood group) antikoerper, C4A antikoerper, C4a antikoerper

Molekulargewicht: 192kDa (predicted)

Gen-ID: 720, 721

Pathways: Komplementsystem