Smooth Muscle Actin Antikörper (N-Term)

Produktnummer ABIN6933907

Produktdetails anti-Smooth Muscle Actin Antikörper

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Bindungsspezifität: N-Term

Reaktivität: Human, Ratte, Maus, Huhn, Rind (Kuh), Kaninchen, Affe, Pavian, Meerschweinchen, Hund, Cat, Schwein, Ziege, Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Smooth Muscle Actin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Spezifität: Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Aufreinigung: Purified by Protein A/G

Immunogen: N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.

Klon: SPM332

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma.

Known Application: Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Andere Bezeichnung: ACTA2 (ACTA2 Produkte)

Synonyme: AAT6 antikoerper, ACTSA antikoerper, MYMY5 antikoerper, GAMMA-ACTIN antikoerper, ACT antikoerper, ACTA3 antikoerper, ACTE antikoerper, ACTL3 antikoerper, ACTSG antikoerper, Act-4 antikoerper, Act4 antikoerper, SMGA antikoerper, 0610041G09Rik antikoerper, Actvs antikoerper, SMalphaA antikoerper, a-SMA antikoerper, alphaSMA antikoerper, wu:fb63d03 antikoerper, ASMA antikoerper, SMactin antikoerper, a-sma antikoerper, aat6 antikoerper, actsa antikoerper, mymy5 antikoerper, sm-actin antikoerper, ACTA2 antikoerper, actc1 antikoerper, ACT-4 antikoerper, asma antikoerper, ACTGE antikoerper, actin, alpha 2, smooth muscle, aorta antikoerper, actin, gamma 2, smooth muscle, enteric antikoerper, actin, alpha 2, smooth muscle, aorta L homeolog antikoerper, ACTA2 antikoerper, ACTG2 antikoerper, Actg2 antikoerper, Acta2 antikoerper, acta2 antikoerper, acta2.L antikoerper

Molekulargewicht: 42kDa

Gen-ID: 59

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development