NFIC Antikörper (AA 323-372)

Produktnummer ABIN6744464

Produktdetails anti-NFIC Antikörper

Target: NFIC (Nuclear Factor I/C (CCAAT-Binding Transcription Factor) (NFIC))

Bindungsspezifität: AA 323-372

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Meerschweinchen, Schwein, Affe, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFIC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human NFIC

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Guinea pig (100%) Chicken (93%) Xenopus (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa323-372 of human NFIC (Q6FI30, NP_995315). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Bat, Pig, Guinea pig (100%), Zebra finch, Chicken (93%), Opossum (92%), Xenopus (86%), Stickleback (80%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Aliquot to Avoid freeze/thaw cycles after reconstitution

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu NFIC

Target: NFIC (Nuclear Factor I/C (CCAAT-Binding Transcription Factor) (NFIC))

Andere Bezeichnung: NFI / NFIC (NFIC Produkte)

Synonyme: LOC100005568 antikoerper, 1110019L22Rik antikoerper, 1500041O16Rik antikoerper, AA589446 antikoerper, AI746521 antikoerper, NF1-C antikoerper, CTF antikoerper, CTF5 antikoerper, NF-I antikoerper, NFI antikoerper, CNFI-C antikoerper, nf1-c1 antikoerper, nuclear factor I/C antikoerper, nuclear factor I C antikoerper, nuclear factor I C L homeolog antikoerper, nfic antikoerper, NFIC antikoerper, Nfic antikoerper, nfic.L antikoerper

Hintergrund: Name/Gene ID: NFIC

Synonyms: NFIC, CTF, CTF5, NF1-C, NF-I/C, NFI, NFI-C, TGGCA-binding protein, NF-I, Nuclear factor 1 C-type, Nuclear factor 1/C, Nuclear factor I/C

Gen-ID: 4782

NCBI Accession: NP_995315

UniProt: P08651