SLC39A5 Antikörper (AA 38-87)

Produktnummer ABIN6741604

Produktdetails anti-SLC39A5 Antikörper

Target: SLC39A5 (Solute Carrier Family 39 (Metal Ion Transporter), Member 5 (SLC39A5))

Bindungsspezifität: AA 38-87

Reaktivität: Human, Rind (Kuh), Hund, Meerschweinchen, Affe, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC39A5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human SLC39A5

Homologie: Percent identity by BLAST analysis: Human (100%) Rat, Rabbit (92%) Dog, Bovine, Pig, Guinea pig (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa38-87 of human SLC39A5 (Q6ZMH5, NP_775867). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Platypus (100%), Galago, Mouse, Rat, Hamster, Elephant, Bat, Rabbit, Horse (92%), Panda, Dog, Bovine, Pig, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu SLC39A5

Target: SLC39A5 (Solute Carrier Family 39 (Metal Ion Transporter), Member 5 (SLC39A5))

Andere Bezeichnung: SLC39A5 / ZIP5 (SLC39A5 Produkte)

Synonyme: 1810013D05Rik antikoerper, 2010205A06Rik antikoerper, Zip5 antikoerper, LZT-Hs7 antikoerper, ZIP5 antikoerper, solute carrier family 39 member 5 antikoerper, solute carrier family 39 (metal ion transporter), member 5 antikoerper, SLC39A5 antikoerper, Slc39a5 antikoerper

Hintergrund: Name/Gene ID: SLC39A5

Synonyms: SLC39A5, LZT-Hs7, ZIP5, Zrt- and Irt-like protein 5, ZIP-5, Zinc transporter ZIP5

Gen-ID: 283375

NCBI Accession: NP_775867

UniProt: Q6ZMH5

Pathways: Transition Metal Ion Homeostasis