ABCA5 Antikörper (AA 1296-1345)

Produktnummer ABIN6741601

Produktdetails anti-ABCA5 Antikörper

Target: ABCA5 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 5 (ABCA5))

Bindungsspezifität: AA 1296-1345

Reaktivität: Human, Hund, Kaninchen, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ABCA5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human ABCA5

Homologie: Percent identity by BLAST analysis: Human (100%) Mouse, Rat (92%) Dog, Rabbit (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa1296-1345 of human ABCA5 (Q8WWZ7, NP_061142). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset (100%), Galago, Mouse, Rat, Panda (92%), Bat (90%), Elephant, Dog, Rabbit, Horse, Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu ABCA5

Target: ABCA5 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 5 (ABCA5))

Andere Bezeichnung: ABCA5 (ABCA5 Produkte)

Synonyme: zgc:163009 antikoerper, ABC13 antikoerper, EST90625 antikoerper, B930033A02Rik antikoerper, mKIAA1888 antikoerper, ATP binding cassette subfamily A member 5 antikoerper, ATP-binding cassette, sub-family A (ABC1), member 5 antikoerper, ATP-binding cassette sub-family A member 5 antikoerper, ABCA5 antikoerper, abca5 antikoerper, LOC100545445 antikoerper, Abca5 antikoerper

Hintergrund: Name/Gene ID: ABCA5
Subfamily: ATP-binding cassette - ABCA/ABC1
Family: Transporter

Synonyms: ABCA5, ATP-binding cassette A5, ABC13, EST90625, KIAA1888

Gen-ID: 23461

NCBI Accession: NP_061142

UniProt: Q8WWZ7