TFAP2C Antikörper (AA 252-301)

Produktnummer ABIN6741554

Produktdetails anti-TFAP2C Antikörper

Target: TFAP2C (Transcription Factor AP-2 gamma (Activating Enhancer Binding Protein 2 Gamma) (TFAP2C))

Bindungsspezifität: AA 252-301

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Zebrafisch (Danio rerio), Affe, Schwein, Kaninchen, Meerschweinchen, Pferd, Schaf, Huhn, Xenopus laevis, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TFAP2C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human TFAP2C / AP2 Gamma

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa252-301 of human TFAP2C (Q92754, NP_003213). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Seabass, Salmon, Stickleback, Zebrafish (100%), Panda, Lizard, Drosophila (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu TFAP2C

Target: TFAP2C (Transcription Factor AP-2 gamma (Activating Enhancer Binding Protein 2 Gamma) (TFAP2C))

Andere Bezeichnung: TFAP2C / AP2 Gamma (TFAP2C Produkte)

Synonyme: erf1 antikoerper, hap-2g antikoerper, tfa2pc antikoerper, tfap2g antikoerper, ap2-gamma antikoerper, TFAP2C antikoerper, AP2-gamma antikoerper, Tcfap2c antikoerper, AP2-GAMMA antikoerper, ERF1 antikoerper, TFAP2G antikoerper, hAP-2g antikoerper, wu:fb48g05 antikoerper, wu:fb49c04 antikoerper, zgc:92088 antikoerper, ap-2g antikoerper, tfap2gamma antikoerper, AA409384 antikoerper, AP2gamma antikoerper, Ap-2.2 antikoerper, Stra2 antikoerper, transcription factor AP-2 gamma antikoerper, putative transcription factor ap-2 gamma antikoerper, transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma) antikoerper, transcription factor AP-2 gamma S homeolog antikoerper, transcription factor AP-2, gamma antikoerper, tfap2c antikoerper, TFAP2C antikoerper, Smp_157840 antikoerper, Tfap2c antikoerper, tfap2c.S antikoerper

Hintergrund: Name/Gene ID: TFAP2C

Synonyms: TFAP2C, AP2 Gamma, AP-2 gamma, ERF-1, ERF1, HAP-2g, Transcription factor ERF-1, TFAP2G, AP2-GAMMA, Estrogen receptor factor 1

Gen-ID: 7022

NCBI Accession: NP_003213

UniProt: Q92754

Pathways: Stem Cell Maintenance