HIF1AN Antikörper (N-Term)

Produktnummer ABIN6736726

Produktdetails anti-HIF1AN Antikörper

Target: HIF1AN (Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN))

Bindungsspezifität: N-Term

Reaktivität: Human, Kaninchen, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HIF1AN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human HIF1AN

Homologie: Percent identity by BLAST analysis: Human (92%) Rabbit, Horse (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human HIF1AN (Q9NWT6, NP_060372). Percent identity by BLAST analysis: Human (100%), Gorilla, Monkey (92%), Gibbon, Marmoset, Elephant, Rabbit, Horse (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu HIF1AN

Target: HIF1AN (Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN))

Andere Bezeichnung: HIF1AN (HIF1AN Produkte)

Synonyme: FIH1 antikoerper, 2310046M24Rik antikoerper, A830014H24Rik antikoerper, cb728 antikoerper, id:ibd5139 antikoerper, hypoxia inducible factor 1 alpha subunit inhibitor antikoerper, hypoxia-inducible factor 1, alpha subunit inhibitor antikoerper, hypoxia inducible factor 1, alpha subunit inhibitor antikoerper, hypoxia-inducible factor 1, alpha subunit inhibitor S homeolog antikoerper, HIF1AN antikoerper, Hif1an antikoerper, hif1an antikoerper, hif1an.S antikoerper

Hintergrund: Name/Gene ID: HIF1AN

Synonyms: HIF1AN, Factor inhibiting HIF-1, FIH1, Factor inhibiting HIF1, FIH-1

Gen-ID: 55662

NCBI Accession: NP_060372

UniProt: Q9NWT6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development