Ziege anti-Maus IgG (Heavy & Light Chain) Antikörper (DyLight 649) - Preadsorbed

Produktnummer ABIN6699023

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: DyLight 649

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Verwendungszweck: Goat Anti Mouse IgG (H&L) DyLight 649™ Conjugated Antibody

Spezifität: This antibody will react with heavy chains of mouse IgG and with light chains of most mouse immunoglobulins.

Produktmerkmale:

Synonyms: Goat Anti Mouse IgG (H&L) DyLight 649™ Conjugated Antibody Pre-Adsorbed, Goat Anti-Mouse IgG Antibody DyLight 649™ Conjugation

Background: Anti-Mouse IgG DyLight 649 Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. 

Immunogen: Mouse IgG whole molecule

Isotyp: IgG

Labeling Ratio: 2.7

Anwendungsinformationen

Applikationshinweise:

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen

Manning, Bundros, Trimmer: "Benefits and pitfalls of secondary antibodies: why choosing the right secondary is of primary importance." in: PLoS ONE, Vol. 7, Issue 6, pp. e38313, (2012) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody