Esel anti-Maus IgG Antikörper (Cy2) - Preadsorbed

Produktnummer ABIN6698985

Produktdetails

Target: IgG

Reaktivität: Maus

Wirt: Esel

Klonalität: Polyklonal

Konjugat: Cy2

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Produktmerkmale:

Synonyms: Donkey Anti-Mouse IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed, Donkey Anti Mouse IgG Antibody CY2 Conjugated

Background: Anti-Mouse IgG Cy2 Antibody generated in donkey detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgG whole molecule

Isotyp: IgG

Labeling Ratio: 3.0

Anwendungsinformationen

Applikationshinweise:

Application Note: Cy2 Conjugated Secondary Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

ELISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

Western Blot Dilution: User Optimized

IF Microscopy Dilution: 1:1,000 - 1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store Cy2 Conjugated Secondary Antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Tibshirani, Tradewell, Mattina, Minotti, Yang, Zhou, Strong, Hayward, Durham: "Cytoplasmic sequestration of FUS/TLS associated with ALS alters histone marks through loss of nuclear protein arginine methyltransferase 1." in: Human molecular genetics, Vol. 24, Issue 3, pp. 773-86, (2015) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody