Kaninchen anti-Maus IgG (Chain gamma 1), (Chain gamma 2a), (Chain gamma 2b), (Chain gamma 3) Antikörper (DyLight 405) - Preadsorbed

Produktnummer ABIN6698971

Produktdetails

Target: IgG

Bindungsspezifität: Chain gamma 1, Chain gamma 2a, Chain gamma 2b, Chain gamma 3

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: DyLight 405

Applikation: ELISA, Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Produktmerkmale:

Synonyms: rabbit anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody DyLight™ 405 conjugation, rabbit anti-Mouse IgG DyLight™405 conjugated Antibody

Background: Anti-Mouse IgG DyLight405 Antibody generated in rabbit detects reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. IgG1, IgG2a, IgG2b and IgG3 chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins

Immunogen Type: Native Protein

Isotyp: IgG

Labeling Ratio: 2.0

Anwendungsinformationen

Applikationshinweise:

Application Note: Mouse IgG subclass pan reactive Secondary Antibody is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Rabbit anti-mouse IgG antibody DyLight™ 405 is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Mouse IgG secondary antibody is available in a variety of formats.

FLISA Dilution: >1:20,000

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen

Zu, Wang, Ping, Sun: "Tan IIA inhibits H1299 cell viability through the MDM4‑IAP3 signaling pathway." in: Molecular medicine reports, Vol. 17, Issue 2, pp. 2384-2392, (2018) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody