Ziege anti-Affe IgG (Chain gamma) Antikörper (DyLight 488) - Preadsorbed

Produktnummer ABIN6698964

Produktdetails

Target: IgG

Bindungsspezifität: Chain gamma

Reaktivität: Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: DyLight 488

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Produktmerkmale:

Synonyms: goat anti-Monkey IgG (gamma chain) DyLight™488 conjugated antibody, goat anti-Monkey IgG gamma antibody DyLight™ 488 conjugation

Background: Anti-Monkey IgG (gamma chain) DyLight™488 conjugated antibody generated in goat detects specifically monkey IgG (gamma chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. This DyLight™488 conjugated anti-Monkey IgG gamma chain secondary antibody is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more general immunoassays. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Anti-Monkey IgG (gamma chain) was produced by repeated immunization with monkey IgG gamma heavy chain in goat.

Immunogen Type: Native Protein

Isotyp: IgG

Labeling Ratio: 5.7

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Monkey IgG (gamma chain) DyLight™488 is suitable for use in immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Specific conditions for reactivity and signal detection should be optimized by the end user.

FLISA Dilution: >1:20,000

Western Blot Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: >1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody