DNMT3A Antikörper (AA 26-100)

Produktnummer ABIN669336

Produktdetails anti-DNMT3A Antikörper

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Bindungsspezifität: AA 26-100

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DNMT3A Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Cow

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Dnmt3a

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-DNMT3A Antikörper (ABIN669336)

Wu, Bu, Yu, Li, Huang, Meng, Zhang, Ma, Li: "Methylation of Septin9 mediated by DNMT3a enhances hepatic stellate cells activation and liver fibrogenesis." in: Toxicology and applied pharmacology, Vol. 315, pp. 35-49, (2016) (PubMed).

Zhao, Hou, Chen, Shao, Zhu, Bu, Gu, Li, Zhang, Du, Fu, Kong, Luo, Long, Li, Deng, Zhao, Cen: "Prenatal cocaine exposure impairs cognitive function of progeny via insulin growth factor II epigenetic regulation." in: Neurobiology of disease, Vol. 82, pp. 54-65, (2015) (PubMed).

Details zu DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Andere Bezeichnung: Dnmt3a (DNMT3A Produkte)

Synonyme: DNMT3A antikoerper, DNMT3A2 antikoerper, M.HsaIIIA antikoerper, MmuIIIA antikoerper, dnmt3a1 antikoerper, dnmt6 antikoerper, DNA methyltransferase 3 alpha antikoerper, DNA methyltransferase 3A antikoerper, DNA (cytosine-5-)-methyltransferase 3 alpha b antikoerper, DNMT3A antikoerper, Dnmt3a antikoerper, dnmt3a antikoerper, dnmt3ab antikoerper

Hintergrund:

Synonyms: TBRS, DNMT3A2, M.HsaIIIA, DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA

Background: Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.

Gen-ID: 1788

UniProt: Q9Y6K1