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Hemagglutinin Antikörper

HA Reaktivität: Influenza A Virus H5N1 ELISA, WB, IP, IHC Wirt: Maus Monoclonal 8A3 unconjugated
Produktnummer ABIN6657567
  • Target Alle Hemagglutinin (HA) Antikörper anzeigen
    Hemagglutinin (HA)
    Reaktivität
    • 66
    • 66
    • 64
    • 39
    • 26
    • 24
    • 16
    • 15
    • 13
    • 8
    • 7
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Influenza A Virus H5N1
    Virus Strain
    A/Vietnam/1203/04
    Wirt
    • 183
    • 137
    • 6
    • 3
    Maus
    Klonalität
    • 182
    • 142
    • 2
    Monoklonal
    Konjugat
    • 251
    • 12
    • 11
    • 9
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 1
    • 1
    Dieser Hemagglutinin Antikörper ist unkonjugiert
    Applikation
    • 243
    • 196
    • 127
    • 116
    • 53
    • 53
    • 45
    • 15
    • 15
    • 10
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
    Aufreinigung
    H5N1 Antibody was purified from tissue culture supernatant fluid by Protein A chromatography and is specific for H5 hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)].  VN04-10 monoclonal antibody did not cross-react with influenza viruses of other HA subtypes. This monoclonal antibody reacted with H5N1 influenza viruses representatives of different clades and subclades of the H5 HA subtype.
    Immunogen

    Immunogen: Hemagglutinin of A/Vietnam/1203/04 Influenza Virus (VN04-10) monoclonal antibody was produced by intraperitoneal immunization of BALB/c mice with concentrated purified virus preparation containing hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)] using the modification of the method described by Kohler and Milstein. Each mouse received two immunizations of 15 mug HA with incomplete Freund's adjuvant, administered 3 week apart.

    Immunogen Type: Native Protein

    Klon
    8A3
    Isotyp
    IgG2b
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  • Applikationshinweise

    Immunohistochemistry Dilution: User Optimized

    Application Note: Hemagglutinin of A/Vietnam/1203/04 Influenza Virus (VN04-10) monoclonal antibody can be used for hemagglutination inhibition (HI) assays to provide antigenic characterization of the influenza A viruses of the H5 HA subtype. This monoclonal antibody is suitable for virus neutralization assays (in cell culture and in embryonated chicken eggs), ELISA, immunoprecipitation, immunohistochemistry and western blotting.

    Neutralization Dilution: User Optimized

    Immunoprecipitation Dilution: User Optimized

    ELISA Dilution: 1:5,000

    Western Blot Dilution: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    0.01 % (w/v) Sodium Azide

    Stabilizer: None

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    RT,4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
  • Reed, Yen, DuBois, Bridges, Salomon, Webster, Russell: "Amino acid residues in the fusion peptide pocket regulate the pH of activation of the H5N1 influenza virus hemagglutinin protein." in: Journal of virology, Vol. 83, Issue 8, pp. 3568-80, (2009) (PubMed).

    Govorkova, Webby, Humberd, Seiler, Webster: "Immunization with reverse-genetics-produced H5N1 influenza vaccine protects ferrets against homologous and heterologous challenge." in: The Journal of infectious diseases, Vol. 194, Issue 2, pp. 159-67, (2006) (PubMed).

    Hanson, Boon, Lim, Webb, Ooi, Webby: "Passive immunoprophylaxis and therapy with humanized monoclonal antibody specific for influenza A H5 hemagglutinin in mice." in: Respiratory research, Vol. 7, pp. 126, (2006) (PubMed).

    Hoffmann, Lipatov, Webby, Govorkova, Webster: "Role of specific hemagglutinin amino acids in the immunogenicity and protection of H5N1 influenza virus vaccines." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 36, pp. 12915-20, (2005) (PubMed).

  • Target
    Hemagglutinin (HA)
    Andere Bezeichnung
    Hemagglutinin (HA Produkte)
    Synonyme
    hemagglutinin antikoerper, Hemagglutinin antikoerper, HA antikoerper, HA antikoerper, ha antikoerper
    Substanzklasse
    Influenza Protein
    Hintergrund

    Synonyms: mouse anti-H5N1 Antibody, mouse anti-VN04-10 Antibody, H5HA antibody, Hemagglutinin 5 antibody, H5N1 antibody

    Background: Hemagglutinin of A/Vietnam/1203/04 Influenza Virus (VN04-10) Antibody raised against the hemagglutinin (HA) surface glycoprotein of the A/Vietnam/1203/04 (H5N1) influenza virus. Generally referred to as ''bird flu'', the H5N1 influenza A virus has been documented in poultry and humans across ten Eurasian countries, from Japan in the north to Indonesia in the south. Without immunity, humans would have no protection against H5N1 influenza viruses, which could potentially cause a catastrophic pandemic influenza.  This antibody, directed against the HA surface glycoprotein of the A/Vietnam/1203/04 (H5N1) influenza virus, is intended to further our understanding of the mechanisms underlying antigenic variation and evolution of novel variants. The major functions of HA include receptor-binding and fusion activities, but there may also be a structural role for HA in viral particle formation. Following attachment of HA to surface receptors on susceptible cells, the influenza virus enters the cell via endocytosis and membrane fusion.

    Gene Name: HA

    Gen-ID
    159144921
    UniProt
    A8UDQ2
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