ABCB11 Antikörper (C-Term)

Produktnummer ABIN6657515

Produktdetails anti-ABCB11 Antikörper

Target: ABCB11 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 11 (ABCB11))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ABCB11 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Aufreinigung: Anti-ABCB11 antibody is directed against human ABCB11 protein.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest reactivity with this protein from human, mouse, and rat based on homology for the immunogen sequence.  Cross reactivity with ABCB11 from other sources has not been determined.

Immunogen:

Immunogen: ABCB11 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human ABCB11, different from the related mouse and rat sequences by three amino acids.

Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 0.5-1 μg/mL

Application Note: Anti-ABCB11 Antibody is useful for Immunohistochemistry -P and Western Blotting. Positive control rat liver, mouse liver, and SMMC whole cell lysate used at 40 μg load and 0.5 μg/mL incubation in western blot and rat mouse, and human liver tissue for IHC-P. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~146.4 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 0.1-0.5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 200μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

0.05 mg Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Details zu ABCB11

Target: ABCB11 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 11 (ABCB11))

Andere Bezeichnung: ABCB11 (ABCB11 Produkte)

Synonyme: ABCB11 antikoerper, Bsep antikoerper, ABC16 antikoerper, BRIC2 antikoerper, BSEP antikoerper, PFIC-2 antikoerper, PFIC2 antikoerper, PGY4 antikoerper, SPGP antikoerper, Lith1 antikoerper, BSEP/SPGP antikoerper, Spgp antikoerper, ATP binding cassette subfamily B member 11 antikoerper, bile salt export pump antikoerper, ATP-binding cassette, sub-family B (MDR/TAP), member 11 antikoerper, ABCB11 antikoerper, LOC100489744 antikoerper, LOC100565276 antikoerper, EDI_111220 antikoerper, EDI_272930 antikoerper, abcb11 antikoerper, Abcb11 antikoerper

Hintergrund:

Synonyms: ATP-binding cassette sub-family B member 11, BSEP, Bile Salt Export Pump

Background: Bile Salt Export Pump (BSEP) is a protein which in humans is encoded by the ABCB 11 gene. It is a member of the superfamily of ATP-binding cassette (ABC) transporters, also known as ABCB11. It is mapped to chromosome 2q24. The BSEP protein is mainly expressed in the liver. ABCB11 is a gene associated with progressive familial intrahepatic cholestasis type 2 (PFIC2) which caused by mutations in the ABCB11 gene will increases the risk of hepatocellular carcinoma in early life. This antibody is suitable for researchers interested in signal transduction and cancer research.

Gene Name: ABCB11

Gen-ID: 8647

NCBI Accession: NP_003733

UniProt: O95342