POLR2A/RPB1 Antikörper (pSer5)

Produktnummer ABIN6655367

Produktdetails anti-POLR2A/RPB1 Antikörper

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Bindungsspezifität: pSer5

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser POLR2A/RPB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Aufreinigung: Anti-Pol II pS5 Antibody was purified by Protein A chromatography. This antibody is specific for Pol II protein phosphorylated Ser5. Cross-reactivity with Pol II pS5 from other sources has not been determined.

Immunogen:

Immunogen: Anti-Pol II S5p Antibody was produced in mice by repeated immunization with the YSPTSPS repeat in the B1 subunit of RNA polymerase II phosphorylated at Ser5 of the repeated sequence.

Immunogen Type: Peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: The rabbit anti-PolII (pSer5) antibody ABIN6655367 is suitable for use in ChIP, CUT&RUN, ELISA, immunofluorescence microscopy, and western blot. Specific conditions for each assay should be optimized by the end user. Expect a band of approximately 225 kDa in western blot in the appropriate cell lysate or extract. General ABIN6655367 dilution recommendations for different applications are as follows:

• ChIP: 1 µg/ChIP
• ELISA: 1:3,000
• IF: 1:500
• WB: 1:1,000
• CUT&RUN: 1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

0.02 % (w/v) Sodium Azide

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Details zu POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Andere Bezeichnung: Pol II (POLR2A Produkte)

Synonyme: NCU02103.1 antikoerper, 19.m02399 antikoerper, POLR2A antikoerper, 220kDa antikoerper, Rpb1 antikoerper, Rpo2-1 antikoerper, POLR2 antikoerper, POLRA antikoerper, RPB1 antikoerper, RPBh1 antikoerper, RPO2 antikoerper, RPOL2 antikoerper, RpIILS antikoerper, hRPB220 antikoerper, hsRPB1 antikoerper, DNA-directed RNA polymerase II largest subunit antikoerper, DNA-directed RNA polymerase II subunit RPB1 antikoerper, RNA polymerase II large subunit Rpb1 antikoerper, RNA polymerase II subunit A antikoerper, DNA-directed RNA polymerase II largest subunit, putative antikoerper, polymerase (RNA) II (DNA directed) polypeptide A, 220kDa antikoerper, polymerase (RNA) II (DNA directed) polypeptide A antikoerper, NCU02103 antikoerper, BBOV_I003360 antikoerper, EDI_169920 antikoerper, CMU_000800 antikoerper, SJAG_01713 antikoerper, MGYG_01527 antikoerper, POLR2A antikoerper, TP03_0185 antikoerper, EBI_22514 antikoerper, CGB_E5020W antikoerper, polr2a antikoerper, Polr2a antikoerper

Hintergrund:

Synonyms: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1

Background: RNA polymerase II (pol II) is a key enzyme in the regulation and control of gene transcription. It is able to unwind the DNA double helix, synthesize RNA, and proofread the result. Pol II is a complex enzyme, consisting of 12 subunits, of which the B1 subunit is the largest. Together with the second largest subunit, B1 forms the catalytic core of the RNA polymerase II transcription machinery. Anti-Pol II S5p Antibody is ideal for research in Gene Expression, Transcription and Genetics.

Gene Name: POLR2A

Gen-ID: 5430

NCBI Accession: NP_000928

UniProt: P24928

Pathways: Regulatorische RNA Pathways