ATP1A2 Antikörper (AA 451-479)

Produktnummer ABIN652011

Produktdetails anti-ATP1A2 Antikörper

Target: ATP1A2 (ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2))

Bindungsspezifität: AA 451-479

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATP1A2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Homologie: B, Pig, Rat

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This ATP1A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 451-479 amino acids from the Central region of human ATP1A2.

Klon: RB19033

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:10~50. WB: 1:2000. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IF)

Validierung #100013 (Immunofluorescence)

by: Memorial Sloan-Kettering Cancer Center, Parada lab

No.: #100013

Datum: 11.05.2016

Antigen: ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2)

Chargennummer: SH09708BF

Validierte Anwendung: Immunofluorescence

Positivkontrolle: Wild-type mouse adult brain cryo-sections

Negativkontrolle: Rabbit IgG

Bewertung: The antibody showed signals along the subventricular zone, which is consistent with the RNA expression analysis.

Validierungsbilder

IF validation of ATP1A2 antibody ABIN652011 on mouse brain sections. A. ABIN652011 diluted 1:10 (red), DAPI counterstaining (blue). B. Dilution factor 1:10. C. Dilution factor 1:50. D. Dilution factor 1:200.

Protokoll

Primärantikörper: ABIN652011

Sekundärantikörper: Donkey-anti-Rabbit-Cy3

Full Protocol:

• Wild-type mouse adult brain was dissected, fixed in 4% PFA for over/night, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
• Immunostaining for Cryo Sections:
• • Dry slides at 50-55°C for 15-30min (or RT for 30min).
  • Wash with PBS 5min X 3 (shake gently).
  • Block with 10% Normal Donkey Serum (NDS) at RT for 1h.
  • Incubate with ABIN652011 diluted 1:10 - 1:800 in 2% NDS at 4°C overnight.
  • Wash with PBS 5min X 3.
  • Incubate with the secondary antibody Donkey-anti-Rabbit-Cy3 together with DAPI 1:100 at RT for 1h (prepared in 2% NDS), cover the chamber with foil.
  • Wash with PBS 5min X 3.
  • Add three drops of water-based Immu-mount.
  • Cover with 24 X 60 cover glass.
• Imaging:
• • Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.

Anmerkungen: ABIN652011 worked at 1:10, did not work well in 1:50-1:200.

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-ATP1A2 Antikörper (ABIN652011)

Du, Kiyoshi, Wang, Wang, Ma, Alford, Zhong, Wan, Chen, Lloyd, Bryan, Zhou: "Genetic Deletion of TREK-1 or TWIK-1/TREK-1 Potassium Channels does not Alter the Basic Electrophysiological Properties of Mature Hippocampal Astrocytes In Situ." in: Frontiers in cellular neuroscience, Vol. 10, pp. 13, (2016) (PubMed).

Wang, Kiyoshi, Du, Ma, Alford, Chen, Zhou: "mGluR3 Activation Recruits Cytoplasmic TWIK-1 Channels to Membrane that Enhances Ammonium Uptake in Hippocampal Astrocytes." in: Molecular neurobiology, (2015) (PubMed).

Details zu ATP1A2

Target: ATP1A2 (ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2))

Andere Bezeichnung: ATP1A2 (ATP1A2 Produkte)

Synonyme: C11orf29 antikoerper, TIF1D antikoerper, TIF1DELTA antikoerper, AW060654 antikoerper, Atpa-3 antikoerper, mKIAA0778 antikoerper, RATATPA2 antikoerper, FHM2 antikoerper, MHP2 antikoerper, atp1a2 antikoerper, atp[a]2 antikoerper, atpa2 antikoerper, cb702 antikoerper, fa10d04 antikoerper, fa10d05 antikoerper, wu:fa10d05 antikoerper, ATP2A antikoerper, atp1a2.L antikoerper, fhm2 antikoerper, mhp2 antikoerper, ATP1A2 antikoerper, ATP1A4 antikoerper, tripartite motif containing 66 antikoerper, ATPase, Na+/K+ transporting, alpha 2 polypeptide antikoerper, ATPase Na+/K+ transporting subunit alpha 2 antikoerper, ATPase, Na+/K+ transporting, alpha 2a polypeptide antikoerper, sodium/potassium-transporting ATPase subunit alpha-2 antikoerper, ATPase, Na+/K+ transporting, alpha 2 polypeptide S homeolog antikoerper, TRIM66 antikoerper, Atp1a2 antikoerper, ATP1A2 antikoerper, atp1a2a antikoerper, atp1a2.S antikoerper, LOC488636 antikoerper

Molekulargewicht: 112265

Gen-ID: 477

NCBI Accession: NP_000693

UniProt: P50993

Pathways: Thyroid Hormone Synthesis, Proton Transport, Ribonucleoside Biosynthetic Process